Stability of genome size among stocks of the channel catfish
Stability of genome size among stocks of the channel catfish
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DOI：
10.1016/0044-8486(90)90207-4
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发表时间：
1990-05
期刊：
影响因子：
4.5
通讯作者：
T. Tiersch;B. A. Simco;K. Davis;R. Chandler;S. Wachtel;G. J. Carmichael
中科院分区：
文献类型：
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作者：
T. Tiersch;B. A. Simco;K. Davis;R. Chandler;S. Wachtel;G. J. Carmichael
用流式细胞仪测定了癍点叉尾（Ictalurus punctatus）雌雄红细胞核DNA含量。研究了14个鲶鱼种群（n=115），包括驯化和野生鱼类，鱼类从性别逆转的人口和那些产生的雌核发育。平均DNA含量为1.977±0.010（SD）pg DNA/细胞，平均贮备液内变异为1.28%。种群与物种平均值的平均差异为0.19%;在种群之间或雄性与雌性之间检测到DNA含量没有显著差异。种内的变化之间的通道鲶鱼股票检查是低于其他鱼类的报告。这可能反映了人为干预的基因组大小的稳定，或者是在癍点叉尾鮰基因组内的进化保守主义。
Nuclear DNA content of erythrocytes from male and female channel catfish (Ictalurus punctatus) was determined by flow cytometry. Fourteen stocks of catfish were studied (n=115), including domesticated and wild fish, fish from sex-reversed populations and those produced by gynogenesis. Mean DNA content was 1.977±0.010 (SD) pg DNA per cell, and mean within-stock variation was 1.28%. The stocks had an average difference of 0.19% from the species mean; no significant differences in DNA content were detected among the stocks or between males and females. The intraspecific variation among the channel catfish stocks examined is lower than that reported for other fish species. This may reflect artificial stabilization of genome size by human intervention, or alternatively, evolutionary conservatism within the genome of the channel catfish.