Reasons for lower transformation efficiency in indica rice using Agrobacterium tumefaciens-mediated transformation: lessons from transformation assays and genome-wide expression profiling
Reasons for lower transformation efficiency in indica rice using Agrobacterium tumefaciens-mediated transformation: lessons from transformation assays and genome-wide expression profiling
复制标题
使用根癌农杆菌介导的籼稻转化效率较低的原因：转化测定和全基因组表达谱分析的经验教训
DOI：
10.1007/s11103-011-9842-5
复制
发表时间：
2012-01-01
影响因子：
5.1
通讯作者：
Lin, Yongjun
中科院分区：
文献类型：
--
作者：
Tie, Weiwei;Zhou, Fei;Lin, Yongjun
根癌农杆菌介导的遗传转化在水稻中已经常规使用了十多年。然而，籼稻品种的转化效率仍不令人满意，远低于粳稻品种。进一步提高转化效率在于植物本身的遗传操作，这需要更好地理解植物细胞对农杆菌感染的易感性的潜在过程以及鉴定参与转化过程的植物基因。在本研究中，不同的粳稻和籼稻品种的瞬时和稳定转化试验表明，籼稻转化效率较低的主要原因是T-DNA整合到植物基因组的效率低。在不同品种的转化过程中的全球基因表达模式的分析显示，主要差异的表达基因响应农杆菌感染后的第一个6小时内，观察到更多的差异表达的基因在籼稻品种珍汕97（ZS），与一些基因在感染早期抑制。基因芯片分析表明，植物的防御反应对农杆菌介导的转化有重要影响。研究表明，在籼稻品种ZS中，一些可能是转化过程中所必需的基因表达下调。该数据集为植物基因组研究提供了一个通用的资源，以了解转化过程中的调控网络，并显示了巨大的希望，提高籼稻转化利用水稻基因组的遗传操作。
Agrobacterium tumefaciens-mediated genetic transformation has been routinely used in rice for more than a decade. However, the transformation efficiency of the indica rice variety is still unsatisfactory and much lower than that of japonica cultivars. Further improvement on the transformation efficiency lies in the genetic manipulation of the plant itself, which requires a better understanding of the underlying process accounting for the susceptibility of plant cells to Agrobacterium infection as well as the identification of plant genes involved in the transformation process. In this study, transient and stable transformation assays using different japonica and indica cultivars showed that the lower transformation efficiency in indica rice was mainly due to the low efficiency in T-DNA integration into the plant genome. Analyses of the global gene expression patterns across the transformation process in different varieties revealed major differences in the expression of genes responding to Agrobacterium within the first 6 h after infection and more differentially expressed genes were observed in the indica cultivar Zhenshan 97 (ZS), with a number of genes repressed early during infection. Microarray analysis revealed an important effect of plant defense response on Agrobacterium-mediated transformation. It has been shown that some genes which may be necessary for the transformation process were down-regulated in the indica cultivar ZS. This dataset provided a versatile resource for plant genomic research to understand the regulatory network of transformation process, and showed great promise for improving indica rice transformation using genetic manipulation of the rice genome.