Identification and partial purification of a factor that stimulates calcium-dependent proteases.
Identification and partial purification of a factor that stimulates calcium-dependent proteases.
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DOI：
10.1021/bi00261a019
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发表时间：
1982-08
期刊：
影响因子：
2.9
通讯作者：
G. Demartino;Donald K. Blumenthal
中科院分区：
文献类型：
--
作者：
G. Demartino;Donald K. Blumenthal
材料和方法大鼠肝脏钙依赖性蛋白酶的制备。如先前所述（DeMartino，1981）制备来自大鼠肝脏的钙依赖性蛋白酶。通过 DE52 离子交换柱色谱分离后，每种蛋白酶均用 50 mM Tris-HCl、1 pH 7.5、0.5 mM 进行透析
Materials and MethodsPreparation of Rat Liver Calcium-Dependent Proteases. Calcium-dependent proteases from rat liver were prepared as described previously (DeMartino, 1981). After isolation by DE52 ion-exchange column chromatography, each protease was dialyzed against 50 mM Tris-HCl, 1 pH 7.5, 0.5 mM