THE RADIATION SENSITIVITY OF NORMAL MOUSE BONE MARROW CELLS, DETERMINED BY QUANTITATIVE MARROW TRANSPLANTATION INTO IRRADIATED MICE
THE RADIATION SENSITIVITY OF NORMAL MOUSE BONE MARROW CELLS, DETERMINED BY QUANTITATIVE MARROW TRANSPLANTATION INTO IRRADIATED MICE
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DOI：
10.2307/3570877
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发表时间：
1960-01-01
影响因子：
3.4
通讯作者：
TILL, JE
中科院分区：
文献类型：
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作者：
MCCULLOCH, EA;TILL, JE
正常小鼠骨髓细胞的辐射敏感性的估计是通过利用以下事实获得的，即静脉注射骨髓后致死辐射小鼠的死亡率降低是注射细胞数量的函数。小鼠经950拉德X线全身照射后，分别注射不同稀释度的骨髓悬液，观察30天的死亡率。使用106个细胞获得最大存活率（80 - 90%），并且随着给予的细胞数量的减少而逐渐降低，当给予104个细胞时降至零。骨髓悬液的照射降低了用106个细胞获得的存活率。正常Swiss或C57 B1小鼠骨髓细胞的辐射敏感性，以将活细胞百分比降低至37%（D37）所需的剂量表示，为105 [±] 24拉德，细胞在体内或体外辐射时具有相似的敏感性。
An estimate of the radiation sensitivity of normal mouse bone marrow cells was obtained by utilizing the fact that the decrease in mortality of lethally irradiated mice following intravenous injection of bone marrow is a function of the number of cells injected. After 950 rads of total-body irradiation with X-rays, groups of 20 mice were injected with different dilutions of marrow suspension and the 30 day mortality was observed. Maximal survival (80 to 90%) was obtained with 106 cells, and decreased progressively as the number of cells administered was decreased, falling to zero when 104 cells were given. Irradiation of the marrow suspension reduced the survival obtained with 106 cells. The radiation sensitivity of normal Swiss or C57 Bl mouse marrow cells, expressed as the dose required to reduce the percentage of viable cells to 37% (D37), is 105 [plus or minus] 24 rads, the cells having a similar sensitivity when irradiated in vivo or in vitro.