The use of germ line-mutated mice in understanding host-pathogen interactions.
The use of germ line-mutated mice in understanding host-pathogen interactions.
复制标题
使用种系突变小鼠来了解宿主与病原体的相互作用。
DOI：
10.1046/j.1462-5822.2002.00224.x
复制
发表时间：
2002
影响因子：
3.4
通讯作者：
Sher,Alan
中科院分区：
文献类型：
--
作者：
Yap,GeorgeS;Sher,Alan
微生物发病机制反映了寄生虫和宿主因素之间的不平衡，这些因素有利于病原体增殖和组织破坏，而不是微生物消除和保存宿主组织完整性所需的因素。宿主-病原体相互作用的体内分析已经彻底改变了设计特定基因改变的能力，包括功能突变的丧失和转基因进入小鼠种系。这篇简短的综述概述了我们所了解的宿主对弓形虫感染的反应，以说明基因改变小鼠在微生物发病机制研究中的作用。考虑缺陷和局限性的实验在敲除小鼠和解决这些问题的方法进行了讨论。最后，介绍了诱导型和组织限制性基因功能改变的研究进展，并对其在微生物学研究中的应用前景进行了展望。
Microbial pathogenesis reflects an imbalance between parasite and host factors that favour pathogen multiplication and tissue destruction over those required for microbial elimination and preservation of the integrity of host tissues.In vivoanalysis of host–pathogen interactions has been revolutionized by the ability to engineer specific genetic alterations including loss of function mutations and transgenes into the mouse germline. This brief review recapitulates what we have learned about the host response toToxoplasma gondiiinfection to illustrate the usefulness of gene‐altered mice in microbial pathogenesis research. A consideration of the pitfalls and limitations of experiments in knockout mice and ways of addressing these concerns are discussed. Finally, advances in inducible and tissue‐restricted alterations in gene function are presented and their possible applications to microbiology research are considered.