Inner hair cell stereocilia are embedded in the tectorial membrane.
Inner hair cell stereocilia are embedded in the tectorial membrane.
复制标题
内毛细胞静纤毛嵌在盖膜中。
DOI：
10.1038/s41467-021-22870-1
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发表时间：
2021-05-10
影响因子：
16.6
通讯作者：
Fridberger A
中科院分区：
文献类型：
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作者：
Hakizimana P;Fridberger A
哺乳动物的听觉依赖于声音诱发的内毛细胞（IHC）静纤毛的移位，这导致内源性机械电换能器通道传导包括Ca 2+在内的阳离子的内向电流。由于它们假定缺乏与覆盖的覆膜（TM）的接触，这些静纤毛的假定刺激机制是通过周围内淋巴的粘性阻力。然而，尽管许多努力，以表征TM的电子显微镜和其他技术，精确的IHC静纤毛TM的关系仍然难以捉摸。在这里，我们发现富含Ca2+的丝状结构，我们称之为Ca2+导管，将TM连接到IHC静纤毛，以使TM能够进行机械刺激，同时确保静纤毛接触TM Ca2+。我们的研究结果要求重新评估的刺激机制的IHC静纤毛和TM在听力中的作用。听力需要内毛细胞（IHC）静纤毛偏转，据信是由于缺乏与覆膜（TM）的接触而引起的流体动力学耦合。在这里，作者表明IHC静纤毛是TM嵌入的，TM中富含钙的结构可能有助于声音传导。
Mammalian hearing depends on sound-evoked displacements of the stereocilia of inner hair cells (IHCs), which cause the endogenous mechanoelectrical transducer channels to conduct inward currents of cations including Ca2+. Due to their presumed lack of contacts with the overlaying tectorial membrane (TM), the putative stimulation mechanism for these stereocilia is by means of the viscous drag of the surrounding endolymph. However, despite numerous efforts to characterize the TM by electron microscopy and other techniques, the exact IHC stereocilia-TM relationship remains elusive. Here we show that Ca2+-rich filamentous structures, that we call Ca2+ ducts, connect the TM to the IHC stereocilia to enable mechanical stimulation by the TM while also ensuring the stereocilia access to TM Ca2+. Our results call for a reassessment of the stimulation mechanism for the IHC stereocilia and the TM role in hearing. Hearing requires inner hair cell (IHC) stereocilia deflection, believed to result from hydrodynamic coupling due to the lack of contact with the tectorial membrane (TM). Here the authors show that IHC stereocilia are TM-embedded, and calcium rich structures in TM may facilitate sound transduction.