CHANGES IN CARTILAGE COMPOSITION AND PHYSICAL-PROPERTIES DUE TO STROMELYSIN DEGRADATION
CHANGES IN CARTILAGE COMPOSITION AND PHYSICAL-PROPERTIES DUE TO STROMELYSIN DEGRADATION
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DOI：
10.1002/art.1780380205
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发表时间：
1995-02-01
影响因子：
--
通讯作者：
GRODZINSKY, AJ
中科院分区：
文献类型：
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作者：
BONASSAR, LJ;FRANK, EH;GRODZINSKY, AJ
目标。目的：研究基质分解素处理对完整软骨移植的生化、组织学和肿胀特性的影响，并将这些影响与组织功能物理特性的变化相关联。牛关节软骨外植体在有或无重组人基质分解素(SLN)的情况下培养长达3天。分别用N-末端测序和Western印迹分析评估和鉴定对基质蛋白多糖和胶原蛋白的损伤。外植体进行力学测试，以评估组织承受循环和静态压缩载荷的能力。SLN处理导致软骨外植体蛋白多糖的时间和剂量依赖性丢失，在暴露于20 NM SLN的3天后显著丢失。组织学显示，蛋白多糖最初的丢失发生在靠近组织表面的区域，并随着SLN暴露时间的增加而向内进行。SLN治疗导致了IX和II型基质胶原的降解，并伴随着组织肿胀的增加。这种基质降解导致组织的功能物理性质的严重变化，包括压缩硬度。结论：高频流动电位法能最准确地检测SLN治疗引起的蛋白多糖的初始局灶性丢失。将完整的软骨暴露于SLN会导致基质分子的特异性、分子水平的降解，从而导致组织的肿胀行为的改变和明显的功能物理特性的恶化。
Objective. To determine the effects of stromelysin treatment on biochemical, histologic, and swelling characteristics of intact cartilage explants and to correlate these effects with changes in the functional physical properties of the tissue.Methods. Bovine articular cartilage explants were cultured for up to 3 days in the presence or absence of recombinant human stromelysin (SLN). Damage to matrix proteoglycans and collagens was assessed and characterized by N-terminal sequencing and Western blot analysis, respectively. Explants were mechanically tested to assess the ability of the tissue to withstand cyclic and static compressive loads.Results. Treatment with SLN resulted in a time- and dose-dependent loss of proteoglycans from cartilage explants, with significant loss seen after 3 days of exposure to 20 nM SLN. Histology indicated that initial loss of proteoglycans occurred in regions near the tissue surface and proceeded inward with increasing time of SLN exposure. SLN treatment resulted in degradation of matrix collagen types IX and II, and a concomitant increase in tissue swelling. This matrix degradation resulted in severe alterations in functional physical properties of the tissue, including compressive stiffness. The initial, focal loss of proteoglycans that resulted from SLN treatment was most accurately detected with high-frequency streaming potential measurements.Conclusion. Exposure of intact cartilage to SLN caused specific, molecular-level degradation of matrix molecules, which resulted in changes in the swelling behavior and marked deterioration of functional physical properties of the tissue.