Improvement of natamycin production by controlling the morphology of Streptomyces gilvosporeus Z8 with microparticle talc in seed preculture
Improvement of natamycin production by controlling the morphology of Streptomyces gilvosporeus Z8 with microparticle talc in seed preculture
复制标题
在种子预培养中用微粒滑石粉控制绿孢链霉菌 Z8 的形态提高那他霉素的产量
DOI：
10.1002/jctb.6668
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发表时间：
2021
影响因子：
3.4
通讯作者：
Feng Li
中科院分区：
文献类型：
--
作者：
Chaoping Yue;Haitao Xu;Yingying Yu;Xin yu;Min Yu;Chen Zhang;Qian You;Shaofan Xia;Zixian Ding;Hao Fu;Xin Zeng;Feng Li
纳他霉素是一种抗真菌药物，已被广泛用作食品防腐剂和治疗真菌疾病的药物。种子预培养在纳他霉素的生产中起着至关重要的作用，但种子形态对纳他霉素生物合成的影响及其调控途径的研究尚不多见，孢子龄、种子形态与纳他霉素产量的相关性分析表明，老孢子易于形成较大的菌丝球，导致纳他霉素生物合成显著降低。为了解决这一问题，在种子预培养过程中加入滑石粉调节菌丝形态。最佳滑石粉添加导致具有毛状表面菌丝体和松散结构的小菌丝球，导致更高的葡萄糖-6-磷酸脱氢酶活性和能荷。种子形态调节有效地提高了纳他霉素滴度并缩短了培养时间，特别是对于老孢子（年龄28天），纳他霉素滴度提高了1.7倍，培养时间缩短了16.9%。不幸的是，直接滑石粉添加证明是不可行的纳他霉素发酵在5 L发酵罐中，这是由于物理损坏快速搅拌和过量的滑石粉粉碎。因此，在种子预培养过程中采用形态学工程策略比常规发酵更适合于纳他霉素的生产。结论本研究提出的新策略不仅可以提高纳他霉素的产量，而且可以缩短培养时间。此外，本研究还为丝状菌或真菌生产其他生物化学品提供了参考，对抗生素工业发酵具有重要意义。© 2021化学工业协会
BACKGROUNDNatamycin, an antifungal agent, has been widely used as a food preservative and medicine for fungal disease therapy. Seed preculture plays a crucial role in natamycin production, while studies on the effects of seed morphology on natamycin biosynthesis and corresponding regulation approaches are still absent.RESULTSCorrelation analyses among spore age, seed morphology and natamycin production showed that old spores tended to form larger mycelial pellets, which gave rise to a significant reduction of natamycin biosynthesis. To solve this problem, microparticle talc was added to regulate the mycelial morphology in seed preculture. Optimal talc addition led to small mycelial pellets with hairy superficial mycelia and loose structure, resulting in higher glucose‐6‐phosphate dehydrogenase activity and energy charge. The seed morphology regulation effectively improved the natamycin titer and decreased the culture time, especially for old spores (age 28 days), with a 1.7‐fold higher natamycin titer and 16.9% culture time reduction. Unfortunately, direct talc addition proved to be infeasible for natamycin fermentation in a 5 L fermentor, which was attributed to physical damage from rapid agitation and excessive talc crashing. Therefore, the morphology engineering strategy was preferred in the seed preculture process rather than formal fermentation for natamycin production.CONCLUSIONThe new strategy proposed in this study could not only improve natamycin production, but also reduced the culture time. Furthermore, this work could provide references for other biochemicals production by filamentous bacteria or fungi, which would be of great significance in industrial antibiotic fermentation. © 2021 Society of Chemical Industry