Plant DNA barcoding: from gene to genome
Plant DNA barcoding: from gene to genome
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DOI：
10.1111/brv.12104
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发表时间：
2015-02-01
影响因子：
10
通讯作者：
Chen, Shilin
中科院分区：
文献类型：
--
作者：
Li, Xiwen;Yang, Yang;Chen, Shilin
DNA条形码目前是一种广泛使用且有效的工具，能够快速、准确地识别植物物种；然而，现有的基因座没有一个适用于所有物种。由于单基因座DNA条形码在近缘分类群中缺乏足够的变异，近期的条形码研究高度重视全叶绿体基因组序列的使用，由于测序技术的改进，这些序列现在更容易获得。虽然叶绿体基因组测序已经能够为准确的植物识别提供可靠的条形码，但它还没有达到资源高效利用的程度，对于非专业实验室设施来说，也还没有提供单基因座条形码那样的分析速度。在此，我们回顾了候选条形码的发展，并讨论了将叶绿体基因组用作超级条形码的可行性。我们倡导一种新的DNA条形码方法，对于选定的分类群组合，将单基因座条形码和超级条形码的最佳使用相结合，以实现高效的植物识别。特定的条形码可能会提高我们在物种和种群水平上区分近缘植物的能力。
DNA barcoding is currently a widely used and effective tool that enables rapid and accurate identification of plant species; however, none of the available loci work across all species. Because single-locus DNA barcodes lack adequate variations in closely related taxa, recent barcoding studies have placed high emphasis on the use of whole-chloroplast genome sequences which are now more readily available as a consequence of improving sequencing technologies. While chloroplast genome sequencing can already deliver a reliable barcode for accurate plant identification it is not yet resource-effective and does not yet offer the speed of analysis provided by single-locus barcodes to unspecialized laboratory facilities. Here, we review the development of candidate barcodes and discuss the feasibility of using the chloroplast genome as a super-barcode. We advocate a new approach for DNA barcoding that, for selected groups of taxa, combines the best use of single-locus barcodes and super-barcodes for efficient plant identification. Specific barcodes might enhance our ability to distinguish closely related plants at the species and population levels.