Adult vascular smooth muscle cells in culture express neural stem cell markers typical of resident multipotent vascular stem cells.
Adult vascular smooth muscle cells in culture express neural stem cell markers typical of resident multipotent vascular stem cells.
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DOI：
10.1007/s00441-014-1937-2
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发表时间：
2014-10
影响因子：
3.6
通讯作者：
Cahill PA
中科院分区：
文献类型：
--
作者：
Kennedy E;Mooney CJ;Hakimjavadi R;Fitzpatrick E;Guha S;Collins LE;Loscher CE;Morrow D;Redmond EM;Cahill PA
在动脉硬化和再狭窄等血管疾病中起主要作用的SMC表型可能与多能血管干细胞(MVSCs)的分化或血管平滑肌细胞(VSMCs)的去分化有关。我们研究了来自三个不同物种(大鼠、小鼠和牛)的vSMCs，以确定它们是否表现出典型的MVSCs的神经干细胞特征。采用免疫细胞化学、共聚焦显微镜、荧光激活细胞分选分析和实时定量聚合酶链式反应等方法，检测其在体外诱导后的SMC分化、神经干细胞标志物表达和多能性。以大鼠主动脉外植体分离的MVSCs、酶分散的大鼠SMC和大鼠骨髓间充质干细胞为对照。小鼠颈动脉裂解物和原代大鼠主动脉VSMCs均为肌球蛋白重链阳性，但弱表达神经脊干细胞标记物Sox10。每株VSMC均表达分化标志物(平滑肌α-肌动蛋白、肌球蛋白重链和钙蛋白)、神经脊干细胞标志物(Sox10+、Sox17+)和胶质细胞标志物(S100β+)。与富含血清的条件相比，血清剥夺显著增加了钙蛋白和肌球蛋白重链的表达，降低了干细胞标记物的表达。VSMCs在成脂或成骨诱导刺激下不能分化为脂肪细胞或成骨细胞，也不能对转化生长因子-β-1或γ-分泌酶抑制后的Notch产生反应。因此，培养中的血管SMCs表达MVSCs特有的神经干细胞标记物，伴随着SMC分化标记物，但不保留其多能性。这些细胞的最终来源可能对它们在体外血管增生性疾病的研究中的应用具有重要意义。
Differentiation of resident multipotent vascular stem cells (MVSCs) or de-differentiation of vascular smooth muscle cells (vSMCs) might be responsible for the SMC phenotype that plays a major role in vascular diseases such as arteriosclerosis and restenosis. We examined vSMCs from three different species (rat, murine and bovine) to establish whether they exhibit neural stem cell characteristics typical of MVSCs. We determined their SMC differentiation, neural stem cell marker expression and multipotency following induction in vitro by using immunocytochemistry, confocal microscopy, fluorescence-activated cell sorting analysis and quantitative real-time polymerase chain reaction. MVSCs isolated from rat aortic explants, enzymatically dispersed rat SMCs and rat bone-marrow-derived mesenchymal stem cells served as controls. Murine carotid artery lysates and primary rat aortic vSMCs were both myosin-heavy-chain-positive but weakly expressed the neural crest stem cell marker, Sox10. Each vSMC line examined expressed SMC differentiation markers (smooth muscle α–actin, myosin heavy chain and calponin), neural crest stem cell markers (Sox10+, Sox17+) and a glia marker (S100β+). Serum deprivation significantly increased calponin and myosin heavy chain expression and decreased stem cell marker expression, when compared with serum-rich conditions. vSMCs did not differentiate to adipocytes or osteoblasts following adipogenic or osteogenic inductive stimulation, respectively, or respond to transforming growth factor-β1 or Notch following γ-secretase inhibition. Thus, vascular SMCs in culture express neural stem cell markers typical of MVSCs, concomitant with SMC differentiation markers, but do not retain their multipotency. The ultimate origin of these cells might have important implications for their use in investigations of vascular proliferative disease in vitro.
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DOI：
10.1007/978-1-61779-523-7_16
发表时间：
2012-01-01
期刊：
CARDIOVASCULAR DEVELOPMENT: METHODS AND PROTOCOLS
影响因子：
--
作者：
Metz, Richard P.;Patterson, Jan L.;Wilson, Emily
通讯作者：
Wilson, Emily
影响因子：
5.2
作者：
John, Nessy;Cinelli, Paolo;Sommer, Lukas
通讯作者：
Sommer, Lukas
影响因子：
5.5
作者：
Guo, Xia;Stice, Steven L.;Chen, Shi-You
通讯作者：
Chen, Shi-You
影响因子：
5
作者：
Gao, Wei;Ferguson, Gail;Cahill, Paul A.
通讯作者：
Cahill, Paul A.
影响因子：
3.7
作者：
Oikawa, Hiroki;Hayashi, Ken'ichiro;Sobue, Kenji
通讯作者：
Sobue, Kenji