A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M1 and Pirlimycin Residues in Milk
A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M1 and Pirlimycin Residues in Milk
复制标题
双色量子点编码熔块免疫测定法用于目视检测牛奶中的黄曲霉毒素 M1 和吡利霉素残留。
DOI：
10.1021/acs.jafc.6b05337
复制
发表时间：
2017-03-01
影响因子：
6.1
通讯作者：
Wang, Xiaomei
中科院分区：
文献类型：
--
作者：
Jiang, Wenxiao;Beloglazova, Natalia V.;Wang, Xiaomei
Mycotoxins and antibacterial agents are the main chemical hazards that lead to several health problems. Nowadays, multiplex immunoassay is a primary goal throughout the world. Here, aflatoxin M1 and pirlimycin were selected as models, and a novel dual colorimetric encoded frit-based immunoassay was developed for simultaneously screening of aflatoxin M1 and pirlimycin residues in milk. This multiplex frit-based immunoassay combined two monoclonal antibodies to extend the spectrum of analytes and to enable detection of two classes of analytes in a single test. The cutoff values were 0.02 μg/kg for aflatoxin M1 and 0.5 μg/kg for pirlimycin, which satisfied the requirement to measure the maximum residue levels. The novel colorimetric frit-based immunoassay has the advantage of high throughput, short analysis time, reduced overall cost per assay, and can be used as a rapid screening technique for simultaneously detecting aflatoxin M1 and pirlimycin residues in milk.
