RNA Interference-Mediated Knockdown of DGAT1 Decreases Triglyceride Content of Bovine Mammary Epithelial Cell Line
RNA Interference-Mediated Knockdown of DGAT1 Decreases Triglyceride Content of Bovine Mammary Epithelial Cell Line
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RNA 干扰介导的 DGAT1 敲除降低牛乳腺上皮细胞系的甘油三酯含量
DOI：
10.3727/105221613x13571653093123
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发表时间：
2012-01-01
期刊：
影响因子：
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通讯作者：
Zhao, Zhihui
中科院分区：
文献类型：
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作者：
Lu, Chunyan;Yang, Runjun;Zhao, Zhihui
二酰基甘油转移酶-1 （DGAT1）在包括乳腺在内的几乎所有组织中表达。缺乏DGAT1的小鼠在乳腺组织中表现出甘油三酯含量降低，并且对饮食诱导的肥胖和糖尿病有抵抗力。因此，DGAT1受到了相当大的关注。本研究采用脂质体介导的RNA干扰（RNAi）技术检测DGAT1在牛乳腺上皮细胞（BMEC）中的功能，并分析DGAT1内源性表达在BMEC中的变化。采用real-time PCR、Western blot、TG kit、紫外分光光度计分别检测RNAi处理前后bmec细胞mRNA和蛋白水平、细胞内甘油三酯（TG）含量、总蛋白含量。结果表明，敲低DGAT1表达可显著降低bmec中TG含量。本研究进一步证实了DGAT1在牛乳腺组织甘油三酯合成中的重要性。
Diacylglyceroltransferase-1 (DGAT1) expresses in nearly all tissues, including the mammary gland. Mice lacking DGAT1 exhibit decreased triglyceride content in mammary tissue, and are resistant to diet-induced obesity and diabetes mellitus. Thus, DGAT1 has received considerable attention. In the present study, the function of DGAT1 was examined by liposome mediated RNA interference (RNAi) to knockdown the expression of endogenous DGAT1 expression in bovine mammary epithelial cells (BMEC) and the changes of the biological functions of cells were analyzed. The mRNA and protein levels, intracellular triglyceride (TG) content, and total protein of BMECs were analyzed by real-time PCR, Western blot, TG kit, and ultraviolet spectrophotometer, respectively, before and after RNAi treatment. The results indicated that knockdown of DGAT1 expression significantly reduced TG content in BMECs. This study further confirmed the importance of DGAT1 in triglyceride synthesis in bovine mammary tissue.