THE T-CELL RECEPTOR-GAMMA CHAIN CD3 COMPLEX - IMPLICATION IN THE CYTOTOXIC ACTIVITY OF A CD3+ CD4- CD8- HUMAN NATURAL-KILLER CLONE
THE T-CELL RECEPTOR-GAMMA CHAIN CD3 COMPLEX - IMPLICATION IN THE CYTOTOXIC ACTIVITY OF A CD3+ CD4- CD8- HUMAN NATURAL-KILLER CLONE
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DOI：
10.1073/pnas.84.11.3861
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发表时间：
1987-06-01
影响因子：
11.1
通讯作者：
SPITS, H
中科院分区：
文献类型：
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作者：
ALARCON, B;DEVRIES, J;SPITS, H
最近已经描述了人类T细胞的子集。这些细胞表达CD 3复合物，但它们不携带经典的T细胞受体（TCR）-α/-。β的异二聚体（WT 31-CD 3+）。相反，它们表达TCR-γ。与另一种称为TCR-δ的多肽结合的链。我们在此报道了具有自然杀伤（NK）样活性的T细胞克隆WM-14具有二硫键桥连的TCR-γ。在其表面上与CD 3缔合的同源二聚体。TCR-γWM-14细胞的多肽链以43、40和38 kDa的三种不同糖基化形式存在，但它们似乎含有相同的多肽骨架。由于WM-14的细胞毒性可被抗CD 3抗体抑制，我们得出结论，TCR-γ- CD 3复合物参与NK样输尿管狭窄杀伤细胞的活性。虽然正常的CD 3-γ，CD3-δ，和CD 3-ε。链存在于该克隆中，与TCR-γ的关联。同源二聚体的存在可能是与CD 3-δ连接的N-连接寡糖完全加工的原因。链
A subset of human T cells has recently been described. These cells express the CD3 complex but they do not carry the classical T-cell receptor (TCR)-.alpha./-.beta. heterodimer on their surface (WT31- CD3+). Instead, they express a TCR-.gamma. chain associated with another type of polypeptide termed TCR-.delta.. We report here that a T-cell clone with natural killer (NK)-like activity, WM-14, had a disulfide bridged TCR-.gamma. homodimer associated with CD3 on its surface. The TCR-.gamma. chains of WM-14 cells were present in three different glycosylation forms of 43, 40, and 38 kDa, but they appeared to contain the same polypeptide backbone. Since cytotoxicity by WM-14 could be inhibited by anti-CD3 antibodies, we conclude that the TCR-.gamma.-CD3 complex was involved in the NK-like urestricted killer activity. Although normal CD3-.gamma., CD3-.delta., and CD3-.epsilon. chains were present in this clone, the association with the TCR-.gamma. homodimer may be the cause of a complete processing of the N-linked oligosaccharides attached to the CD3-.delta. chain.