RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases
RNA-dependent chromatin association of transcription elongation factors and Pol II CTD kinases
复制标题
DOI：
10.7554/elife.25637
复制
发表时间：
2017-05-24
期刊：
影响因子：
7.7
通讯作者：
Cramer, Patrick
中科院分区：
文献类型：
--
作者：
Battaglia, Sofia;Lidschriber, Michael;Cramer, Patrick
对于通过染色质的转录，RNA聚合酶（Pol）II与延伸因子（EF）缔合。在这里，我们表明，许多EFs交联的RNA出现从转录Pol II在酵母酿酒酵母。大多数EF优先与mRNA交联，而不是不稳定的非编码RNA。RNA有助于许多EF的染色质缔合，包括Pol II丝氨酸2激酶Ctkl和Burl以及组蛋白H3甲基转移酶Setl和Set 2。Ctkl激酶复合物在体外结合RNA，与直接EF-RNA相互作用一致。Setl在体内向基因的募集依赖于其RNA识别基序（RRM）。这些结果强烈表明，新生RNA有助于EF募集转录Pol II。我们提出，EFRNA相互作用促进组装的延伸复合物的转录基因时，RNA出现从Pol II，和EF-RNA相互作用的损失后，在多聚腺苷酸化位点的RNA切割触发拆卸的延伸复合物。
For transcription through chromatin, RNA polymerase (Pol) II associates with elongation factors (EFs). Here we show that many EFs crosslink to RNA emerging from transcribing Pol II in the yeast Saccharomyces cerevisiae. Most EFs crosslink preferentially to mRNAs, rather than unstable non-coding RNAs. RNA contributes to chromatin association of many EFs, including the Pol II serine 2 kinases Ctkl and Burl and the histone H3 methyltransferases Setl and Set2. The Ctkl kinase complex binds RNA in vitro, consistent with direct EF-RNA interaction. Setl recruitment to genes in vivo depends on its RNA recognition motifs (RRMs). These results strongly suggest that nascent RNA contributes to EF recruitment to transcribing Pol II. We propose that EFRNA interactions facilitate assembly of the elongation complex on transcribed genes when RNA emerges from Pol II, and that loss of EF-RNA interactions upon RNA cleavage at the polyadenylation site triggers disassembly of the elongation complex.