H-NS represses transcription of the flagellin gene lafA of lateral flagella in Vibrio parahaemolyticus
H-NS represses transcription of the flagellin gene lafA of lateral flagella in Vibrio parahaemolyticus
复制标题
H-NS抑制副溶血弧菌侧鞭毛鞭毛蛋白基因lafA的转录
DOI：
10.1139/cjm-2017-0315
复制
发表时间：
2018
影响因子：
2.8
通讯作者：
Wenhui Yang
中科院分区：
文献类型：
--
作者：
Yan Wang;Yiquan Zhang;Zhe Yin;Jie Wang;Yongzhe Zhu;Haoran Peng;Dongsheng Zhou;Zhongtian Qi;Wenhui Yang
群居运动最终是由副溶血性弧菌质子驱动的外侧鞭毛系统介导的。flafgenes的表达受到许多环境条件和调控因子的严格调控。核苷相关的dna结合蛋白H-NS是一种小而丰富的蛋白，广泛分布于细菌中，已在霍乱弧菌和v中鉴定出H-NS样蛋白依赖于flflgenes的表达。parahaemolyticus。本研究的数据表明，H-NS可抑制副溶血性细菌的群体运动。检测到一个单一的σ28依赖性启动子编码外侧鞭毛蛋白，其活性被H-NS直接抑制。因此，H-NS通过直接作用于α抑制蜂群运动。简而言之，这项工作揭示了H-NS作为副溶血性细菌flafa表达和群体运动的抑制因子的新功能。
Swarming motility is ultimately mediated by the proton-powered lateral flagellar (laf) system inVibrio parahaemolyticus. Expression oflafgenes is tightly regulated by a number of environmental conditions and regulatory factors. The nucleoid-associated DNA-binding protein H-NS is a small and abundant protein that is widely distributed in bacteria, and H-NS-like protein-dependent expression oflafgenes has been identified inVibrio choleraeandV. parahaemolyticus. The data presented here show that H-NS acts as a repressor of the swarming motility inV. parahaemolyticus. A single σ28-dependent promoter was detected forlafAencoding the flagellin of the lateral flagella, and its activity was directly repressed by H-NS. Thus, H-NS represses swarming motility by directly acting onlafA. Briefly, this work revealed a novel function for H-NS as a repressor of the expression oflafAand swarming motility inV. parahaemolyticus.