Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines.
Monoclonal antibodies specific for carcinoembryonic antigen and produced by two hybrid cell lines.
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癌胚抗原特异性单克隆抗体，由两种杂交细胞系产生。
DOI：
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发表时间：
1980
影响因子：
11.1
通讯作者：
J. Mach
中科院分区：
文献类型：
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作者：
R. Accolla;S. Carrel;J. Mach
用纯化的癌胚抗原（CEA）免疫小鼠脾脏细胞，将其与小鼠骨髓瘤细胞系P3-NSI/1-Ag4融合。在获得的400个杂交种中，2个分泌抗体特异性地与存在于CEA分子上的两种不同抗原决定因子反应。它们被克隆并建立为永久杂交瘤细胞系。这些抗体具有相对高的亲和力，可以无限量生产，将用于CEA的免疫化学表征，并作为鉴定人体组织和体液中该抗原的标准试剂。
Spleen cells from mice immunized with purified carcinoembryonic antigen (CEA), an important tumor marker of human carcinomas, were fused with the mouse myeloma cell line P3-NSI/1-Ag4. Out of the 400 hybrids obtained, 2 secreted antibodies reacting specifically with two different antigenic determinants present on CEA molecules. They were cloned and established as permanent hybridoma cell lines. These antibodies, which have relatively high-affinities and can be produced in unlimited amounts, will be useful both for the immunochemical characterization of CEA and as a standard reagent for the identification of this antigen in human tissues and body fluids.