Time-dependent speckle in holographic optical coherence imaging and the health of tumor tissue.
Time-dependent speckle in holographic optical coherence imaging and the health of tumor tissue.
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DOI：
10.1364/ol.29.000068
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发表时间：
2004
期刊：
影响因子：
3.6
通讯作者：
P. Yu;L. Peng;M. Mustață;J. Turek;M. Melloch;D. Nolte
中科院分区：
文献类型：
--
作者：
P. Yu;L. Peng;M. Mustață;J. Turek;M. Melloch;D. Nolte
全息光学相干成像从散射组织内部的连续深度获取面部图像。在多细胞肿瘤球体的研究中，从固定深度记录的全息特征被观察到是时间相关的，它们可以被分类为可变的或持久的。可变特征与持久特征的比率以及散斑相关时间提供了对组织健康状况的定量测量。对受到代谢和交叉聚合毒物影响的大鼠骨肉瘤肿瘤球体的研究提供了健康、坏死和中毒组织之间的定量区分。健康组织中细胞器的运动表现为超布朗激光散斑，而化学固定的组织则表现为静态散斑。
Holographic optical coherence imaging acquires en face images from successive depths inside scattering tissue. In a study of multicellular tumor spheroids the holographic features recorded from a fixed depth are observed to be time dependent, and they may be classified as variable or persistent. The ratio of variable to persistent features, as well as speckle correlation times, provides quantitative measures of the health of the tissue. Studies of rat osteogenic sarcoma tumor spheroids that have been subjected to metabolic and cross-polymerizing poisons provide quantitative differentiation among healthy, necrotic, and poisoned tissue. Organelle motility in healthy tissue appears as super-Brownian laser speckle, whereas chemically fixed tissue exhibits static speckle.