Optical tweezers integrated surface plasmon resonance holographic microscopy for characterizing cell-substrate interactions under noninvasive optical force stimuli
Optical tweezers integrated surface plasmon resonance holographic microscopy for characterizing cell-substrate interactions under noninvasive optical force stimuli
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DOI：
10.1016/j.bios.2022.114131
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发表时间：
2022-03-04
影响因子：
12.6
通讯作者：
Zhao, Jianlin
中科院分区：
文献类型：
--
作者：
Dai, Siqing;Mi, Jingyu;Zhao, Jianlin
随着生物力学研究的迅速发展，对力作用下基底附近细胞行为的实时研究变得越来越重要。在这里，我们提出了一个光镊（OT）集成表面等离子体共振全息显微镜（SPRHM），实现动态和原位表征的细胞与基板的相互作用与非侵入性的光力刺激。利用OT集成SPRHM（OT-SPRHM）对活细胞进行了动态操纵，同时获得了活细胞的相衬表面等离子体共振图像，并通过SPRHM确定了细胞-基底距离。我们表明，OT-SPRHM具有先进的测量光学力的能力，其微小的变化施加到K562细胞附近的基板。此外，我们首次揭示了OT对MC 3 T3-E1细胞的操纵作用。证明其鲁棒性，这种技术提供了一个强大的工具，探索各种生物样品的响应力刺激沿着细胞-基板界面的生物传感区域。
The rapid development of bio-mechanical research increases the significance of studying cell behaviors near the substrate under the force stimuli in a real-time manner. Here, we present an optical tweezers (OT) integrated surface plasmon resonance holographic microscopy (SPRHM) to realize the dynamical and in-situ characterizations of cell-substrate interactions with noninvasive optical force stimulations. Using the OT integrated SPRHM (OT-SPRHM), we dynamically manipulate the living cells by OT, and simultaneously, the phase-contrast surface plasmon resonance images of the living cells are obtained and the cell-substrate distance is determined via SPRHM. We show that OT-SPRHM has the advanced capabilities of measuring the optical force and its tiny variations applied to the K562 cells near the substrate. Also, we for the first time reveal the manipulation of the MC3T3-E1 cells by OT. Demonstrating its robustness, this technique provides a powerful tool to explore the responses of various biological specimens to the force stimuli along the cell-substrate interface in the bio-sensing area.