Identification of salsolinol in the mediobasal hypothalamus of lactating ewes and its relation to suckling-induced prolactin and GH release.
Identification of salsolinol in the mediobasal hypothalamus of lactating ewes and its relation to suckling-induced prolactin and GH release.
复制标题
哺乳期母羊下丘脑内侧基底层中猪油醇的鉴定及其与哺乳诱导的催乳素和 GH 释放的关系。
作者：
T. Misztal;K. Górski;D. Tomaszewska;E. Molik;K. Romanowicz
在泌乳母羊（n = 7）中进行漏斗核正中隆起（IN/ME）的推拉灌注两次，以鉴定多巴胺（DA）衍生的猪毛菜醇及其细胞外浓度的变化。每只母羊的灌流液收集期包括对照非哺乳期1000 - 1230 h（5次灌流）和哺乳期1230 - 1500 h（随后5次灌流）。同时，从1000至1500小时，每隔10分钟采集一次血样。用HPLC法测定灌流液中猪毛菜醇和DA的浓度，用RIA法测定血浆中催乳素和GH的浓度。与非哺乳期相比，哺乳期从IN/ME前部和后部收集的灌注液中的猪毛菜醇平均浓度（根据灌注部位的死后定位）显著增加（分别为P <0.05和P <0.001）。虽然没有发现DA的前部，只有残留量的DA被发现在一些灌注液收集的后部。在断奶后10周（季节性不发情）的IN/ME母羊中未检测到猪毛菜醇。哺乳期的平均血浆催乳素和GH浓度显著（P <0.001）高于非哺乳期。总之，我们目前的研究表明，猪毛菜醇存在于泌乳母羊的IN/ME中，其细胞外浓度在哺乳期间增加。此外，它支持的作用，猪毛菜醇作为一种神经递质参与的催乳素分泌的调节过程中，至少在哺乳期。
The push-pull perfusions of the infundibular nucleus-median eminence (IN/ME) were made in lactating ewes (n=7) twice, to identify dopamine (DA)-derived salsolinol and the changes in its extracellular concentration in response to suckling. The perfusate collecting period in every ewe consisted of control non-suckling period, 1000-1230 h (five perfusates), and suckling period, 1230-1500 h (next five perfusates). Simultaneously, blood samples were collected from 1000 to 1500 h at 10-min intervals. The perfusate concentrations of salsolinol and DA were measured by HPLC, and plasma prolactin and GH concentrations were assayed by the RIA. Mean concentrations of salsolinol in perfusates collected from the anterior and posterior parts of the IN/ME (according to post-mortem localization of a perfusion site) increased significantly (P<0.05 and P<0.001 respectively) during the suckling period, when compared with those noted during the non-suckling period. While no DA was found in the anterior part, only vestigial amounts of DA were found in a few perfusates collected from the posterior part. Salsolinol was not detected in the IN/ME of ewes 10 weeks after weaning (seasonal anoestrus). Mean plasma prolactin and GH concentrations during suckling were significantly (P<0.001) higher than those noted during the non-suckling period. In conclusion, our current study reveals that salsolinol is present in the IN/ME of lactating ewes and that its extracellular concentration increases during suckling. Moreover, it supports the role of salsolinol as a neurotransmitter involved in the regulatory process of prolactin secretion at least during lactation.
阿片类药物对大鼠哺乳期间垂体前叶激素反应的调节。
影响因子：
4.8
作者：
Riskind,PN;Millard,WJ;Martin,JB
通讯作者：
Martin,JB
大鼠泌乳早期和中期期间，结节漏斗部多巴胺能神经元对催乳素反馈的反应性减弱。
DOI：
10.1210/endo.137.1.8536641
发表时间：
1996
期刊：
Endocrinology.
影响因子：
--
作者：
Arbogast,LA;Voogt,JL
通讯作者：
Voogt,JL