A method for quantitative extraction of enzymes and metabolites from tissue samples in the milligram range.
A method for quantitative extraction of enzymes and metabolites from tissue samples in the milligram range.
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一种从组织样品中定量提取毫克范围内的酶和代谢物的方法。
DOI：
10.1177/30.4.7061832
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
H. Reichmann
中科院分区：
文献类型：
--
作者：
D. Pette;H. Reichmann
本文描述了一种从组织样品中定量提取毫克范围内可溶性酶活性的方法。冷冻组织的机械崩解在微型研钵中进行，粉末状组织可从微型研钵中定量转移，用于在微量离心管中以小体积提取。
A method is described for quantitative extraction of soluble enzyme activities from tissue samples in the milligram range. Mechanical disintegration of the frozen tissue is performed in a micromortar from which the powdered tissue can be quantitatively transferred for extraction in small volumes within a microcentrifuge tube.