Fluorescence and Electron Microscopy Methods for Exploring Antimicrobial Peptides Mode(s) of Action
Fluorescence and Electron Microscopy Methods for Exploring Antimicrobial Peptides Mode(s) of Action
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DOI：
10.1007/978-1-60761-594-1_16
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发表时间：
2010-01-01
期刊：
影响因子：
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通讯作者：
Mangoni, Maria Luisa
中科院分区：
文献类型：
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作者：
Marcellini, Ludovica;Giammatteo, Maria;Mangoni, Maria Luisa
由于微生物病原体对现有药物的耐药性不断增加，迫切需要鉴定具有新作用机制的新型抗菌药物。在这种情况下，阳离子抗菌肽（AMP）被认为是有前途的候选者。尽管有证据表明，与传统抗生素相比，微生物膜是大量AMIN的主要靶点，从而使病原体难以获得耐药性，但其作用方式尚不完全清楚。目前，大量研究致力于了解 AMP 在亚致死或致死肽浓度下对完整细胞的影响，荧光/电子显微镜技术是深入了解这些分子对靶细胞形态和膜结构造成的损伤的有效工具。我们在这里概述了解决这个问题的一些微观方法。
Due to the increasing resistance of microbial pathogens to the available drugs, the identification of new antimicrobial agents with a new mechanism of action is urgently needed. In this context, cationic antimicrobial peptides (AMPs) are considered promising candidates. Although there is evidence that, in contrast to conventional antibiotics, microbial membranes are the principal target of large number of AMIN, thus making it difficult for the pathogen to acquire resistance, their mode(s) of action is not yet completely clear. Intense research is currently devoted to understand the effect(s) of AMPs on intact cells, either at sub-lethal or at lethal peptide concentrations, and fluorescence/electron microscopy techniques represent a valid tool to get insight into the damage caused by these molecules on the morphology and membrane structure of the target cell. We here present an overview of some microscopic methodologies to address this issue.