INHIBITION OF SERUM-ALBUMIN FLUX ACROSS EXPOSED DENTIN FOLLOWING CONDITIONING WITH GLUMA PRIMER, GLUTARALDEHYDE OR POTASSIUM OXALATES
INHIBITION OF SERUM-ALBUMIN FLUX ACROSS EXPOSED DENTIN FOLLOWING CONDITIONING WITH GLUMA PRIMER, GLUTARALDEHYDE OR POTASSIUM OXALATES
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DOI：
10.1016/0300-5712(93)90133-b
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发表时间：
1993-08-01
影响因子：
4.4
通讯作者：
KNUTSSON, G
中科院分区：
文献类型：
--
作者：
BERGENHOLTZ, G;JONTELL, M;KNUTSSON, G
在各种治疗程序中用于牙本质的各种药物可能会导致牙本质渗透性降低。通过观察内源性血清白蛋白穿过暴露的牙本质的流动，评估已知促进修复树脂的牙本质粘合或延迟牙本质敏感性的试剂关于其阻止牙本质流体流动的能力。在年轻成年猕猴中进行实验，在门牙和犬齿中使用V类腔制备物。凝胶扩散-酶联免疫吸附试验（DIG-ELISA）用于定量在不处理或局部应用GLUMA引物、戊二醛或辛酸钾后不同时间段这些腔流出物中的血清白蛋白。虽然在未处理的牙洞中，血清白蛋白甚至在1周后仍继续流动，但在局部应用所测试的试剂后观察到血清白蛋白流量的显著减少或完全停止，表明对牙本质流体流动的持久作用。未观察到药物之间的差异。进一步切割腔底零点几毫米，导致血清白蛋白重新流动。数据表明，测试的溶液能够减少牙本质流体流到暴露的牙本质表面上。
A variety of medicaments used on dentine in various treatment procedures may cause a reduction in dentine permeability. By observing the flow of endogenous serum albumin across exposed dentine, agents known to promote dentine bonding of restorative resins or retard dentine sensitivity were assessed regarding their capacity to arrest dentinal fluid flow. Experiments were conducted in young adult macaque monkeys employing Class V cavity preparations in incisors and canines. A diffusion-in-gel-enzyme-linked-immunosorbent-assay (DIG-ELISA) was used to quantitate serum albumin in effluents from these cavities at various time periods following either no treatment or topical application with GLUMA primer, glutaraldehyde or potassium oxalates. While in untreated cavities serum albumin continued to flow even after a period of 1 week, a substantial reduction or complete cessation of serum albumin flux was seen following topical application of the agents tested, suggesting a durable effect on dentinal fluid flow. No difference between agents was observed. Further cutting of the cavity bottom a few tenths of a millimetre resulted in renewed flow of serum albumin. Data suggest that the solutions tested are capable of reducing dentinal fluid flow onto exposed dentine surfaces.