HLA-A2-Restricted Epitopes Identified from MTA1 Could Elicit Antigen-Specific Cytotoxic T Lymphocyte Response.
HLA-A2-Restricted Epitopes Identified from MTA1 Could Elicit Antigen-Specific Cytotoxic T Lymphocyte Response.
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从 MTA1 鉴定出的 HLA-A2 限制性表位可引发抗原特异性细胞毒性 T 淋巴细胞反应。
DOI：
10.1155/2018/2942679
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Gao Yanfeng
中科院分区：
文献类型：
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作者：
Wu Yahong;Zhai Wenjie;Zhou Xiuman;Wang Zhiwei;Lin Yan;Ran Ling;Qi Yuanming;Gao Yanfeng
肿瘤转移相关蛋白1(MTA1)在多种人类恶性肿瘤中均有过表达，与肿瘤侵袭转移、放疗和化疗耐药密切相关，是理想的候选肿瘤抗原。我们在MTA1中鉴定了几个人类白细胞抗原A2限制性表位，并评价了它们与人类白细胞抗原∗0201分子的结合能力。随后，表达了包含优势表位MTA1(1-283)的重组片段，并检测了所选表位和MTA1(1-283)片段诱导细胞毒性T淋巴细胞(CTL)的能力。我们的结果表明，所获得的表位和MTA1(1-283)片段在体内外均能诱导人类白细胞抗原A2限制性和抗原特异性的CTL反应。本文确定的新表位可能有助于促进针对表达MTA1的人类白细胞抗原A2+患者的新型治疗性疫苗的开发。
Overexpression of metastasis‐associated protein 1 (MTA1) has been observed in many human malignancies and is significantly related to tumor invasion and metastasis, therapeutic resistance to radiation and chemotherapy, making MTA1 an ideal candidate tumor antigen. We identified several human leukocyte antigen‐ (HLA‐) A2‐restricted epitopes in MTA1 and evaluated their binding ability to HLA‐A∗0201 molecules. Subsequently, a recombinant fragment encompassing the dominant epitopes, MTA1(1–283), was expressed, and the abilities of the selected epitopes of MTA1 and the MTA1(1–283)fragment to induce cytotoxic T lymphocytes (CTLs) were examined. Our results indicated that the epitopes and MTA1(1–283)fragment elicited HLA‐A2‐restricted and antigen‐specific CTL responses bothin vitroandin vivo. The new epitopes identified here may help promote the development of new therapeutic vaccines for HLA‐A2+patients expressing MTA1.