Ectopic overexpression of AtHDG11 in tall fescue resulted in enhanced tolerance to drought and salt stress
Ectopic overexpression of AtHDG11 in tall fescue resulted in enhanced tolerance to drought and salt stress
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DOI：
10.1007/s00299-008-0659-x
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发表时间：
2009-04-01
影响因子：
6.2
通讯作者：
Kuai, Ben-Ke
中科院分区：
文献类型：
--
作者：
Cao, Ya-Jun;Wei, Qiang;Kuai, Ben-Ke
高羊茅（Festuca arundinacea Schreb.）是一种冷季型多年生草，通常在温带地区种植。然而，作为冷季型草坪草的主要类型，其生长已扩展到亚热带气候甚至亚热带和热带之间的过渡气候，并且在某些情况下，严重盐渍化的土地。长期的生长对它对非生物胁迫的耐受性提出了严峻的挑战，特别是对干旱、盐和高温的耐受性。在这里，我们报告了一个成功的引进拟南芥AtHDG 11到高羊茅通过农杆菌介导的转化。AtHDG 11在CaMV 35 S启动子控制下的异位过表达导致对干旱和盐胁迫的耐受性显著增强。对转基因植株的生长发育没有明显的不良影响。在干旱和/或盐胁迫条件下，转基因植株根系发达，丙二醛水平降低，Na+/K+比值接近正常，脯氨酸水平升高，SOD和CAT活性的诱导增强，表明活性氧清除能力增强可能在获得非生物胁迫耐受性中起重要作用。
Tall fescue (Festuca arundinacea Schreb.) is a cool-season perennial grass, which has been conventionally grown in the temperate area. However, as a major type of cool-season turf grass, its growth has been extended to the sub-tropical climate or even to the transitional climate between the sub-tropical and the tropical, and, in some cases, to heavily salinized lands. The extended growth imposes a serious challenge to its tolerance to the abiotic stress, particularly to drought, salt and high temperature. Here, we report a successful introduction of Arabidopsis AtHDG11 into the tall fescue via Agrobacterium-mediated transformation. The ectopic overexpression of AtHDG11 under the control of CaMV 35S promoter with four enhancers resulted in significantly enhanced tolerance to drought and salt stress. No obvious adverse effects on growth and development were observed in the transgenic plants. The enhanced stress tolerance was associated with a more extensive root system, a lower level of malondialdehyde, a nearly normal Na+/K+ ratio, a higher level of proline and a kinetically accelerated induction of SOD and CAT activities observed in the transgenic plants during drought and/or salt stress, indicating that an enhanced ROS scavenging capability might play a significant role in the acquired tolerance to the abiotic stress.