Interferon-gamma serum level and immunohistochemical expression ofCD8cells in tissue biopsies in patients with alopecia areata in correlation with trichoscopic findings
Interferon-gamma serum level and immunohistochemical expression ofCD8cells in tissue biopsies in patients with alopecia areata in correlation with trichoscopic findings
复制标题
DOI：
10.1111/dth.13718
复制
发表时间：
2020-07-05
影响因子：
3.6
通讯作者：
Abozeid, Sami
中科院分区：
文献类型：
--
作者：
Agamia, Naglaa;Apalla, Zoe;Abozeid, Sami
斑秃（AA）是一种自身免疫性非瘢痕性脱发。本研究的目的是评估血清干扰素-γ（IFN-γ）浓度和病变皮肤活检组织中CD 8细胞表达与AA患者疾病严重程度、活动性、持续时间和内窥镜检查结果的相关性。该研究包括30名AA患者和15名年龄和性别匹配的健康对照。进行毛发镜检查并拍摄脱发区域的照片，并使用酶联免疫吸附测定技术进行血清IFN-γ水平评估和CD 8细胞免疫组化。结果表明，患者血清IFN-γ水平显著高于对照组，并与活动状态和病程显著相关。CD 8 + T细胞浸润强度与病情严重程度显著相关。黄点（YD）、毫毛、黑点和感叹号是最常见的内窥镜检查结果。黑点的存在与疾病活动性、持续时间、血清IFN-γ和CD 8+浸润强度显著相关。YD的存在与平均血清IFN-γ水平显著相关。感叹号与疾病活动性和CD 8+浸润程度显著相关。结论：关节镜检查可作为反映再障患者血清IFN-γ水平和CD 8 + T细胞浸润强度的可靠指标。
Alopecia areata (AA) is an autoimmune form of nonscarring hair loss. The aim of the study was to assess the serum concentration of interferon gamma (IFN-gamma) and CD8 cell expression in lesional skin biopsies in correlation with the disease severity, activity, duration, and trichoscopic findings in patients with AA. The study included 30 patients with AA and 15 age- and sex-matched healthy controls. Trichoscopy was performed and photographs were captured for the alopecic areas, and the enzyme-linked immunosorbent assay technique was used for serum level of IFN-gamma assessment and immunohistochemistry for CD8 cells. The results obtained indicate that IFN-gamma serum level in patients was significantly higher than that of control subjects, and significantly correlated with the activity status and the duration of the disease. CD8+ T cells infiltrate intensity significantly correlated with severity. Yellow dots (YDs), vellus hair, black dot, and exclamation marks were the most common trichoscopic findings. The presence of black dots significantly correlated to the disease activity, duration, serum IFN-gamma, and CD8+ infiltrate intensity. The presence of YDs significantly correlated with the mean serum IFN-gamma level. Exclamation marks significantly correlated with the disease activity and the degree of CD8+ infiltrate. In conclusion, trichoscopy could be a reliable indicator of the IFN-gamma serum level and CD8+ T cell infiltrate intensity in AA patient.