Hormonal regulation of phase polymorphism and storage-protein fluctuation in the common cutworm, Spodoptera litura
Hormonal regulation of phase polymorphism and storage-protein fluctuation in the common cutworm, Spodoptera litura
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DOI：
10.1016/0022-1910(85)90004-6
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发表时间：
1985
影响因子：
2.2
通讯作者：
S. Tojo;M. Morita;N. Agui;K. Hiruma
中科院分区：
文献类型：
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作者：
S. Tojo;M. Morita;N. Agui;K. Hiruma
通过抗蛹脂肪体蛋白的抗血清检测到，斜纹夜蛾最后龄幼虫合成了三种储存蛋白。假定的蛹储存蛋白 1 和 2 在拥挤饲养条件下蜕皮后 36 小时出现在末龄幼虫的血淋巴中：在隔离条件下，它们在 1 天后出现。保幼激素治疗可以阻止这些蛋白质在血淋巴中的出现，并可以通过全乳腺切除术来增强。将 20-羟基蜕皮酮注射到结扎的幼虫中不会诱导这两种蛋白质的出现。与家蚕抗血清反应的蛋白 3 的积累不会被保幼激素阻断，并且在两个阶段中是相似的。在蜕皮至最后幼虫龄期间，它还在血淋巴中少量积累，然后在蜕皮时消失。 100 ng/ml 的蜕皮类固醇导致这些蛋白质被脂肪体隔离，但较高浓度的蜕皮类固醇（200 ng/ml）在离体腹部产生蛹角质层，表明这两个事件需要不同的蜕皮类固醇浓度。
Three storage proteins are synthesised bySpodoptera lituralast-instar larvae as detected by an antiserum against pupal fat body proteins. The putative pupal storage proteins 1 and 2, appear in the haemolymph of the last-instar larvae 36 h after ecdysis under crowded rearing conditions: they appear 1 day later in isolated conditions. The appearance of these proteins in the haemolymph is prevented by juvenile hormone treatment and enhanced by allatectomy. Injection of 20-hydroxyecdysone into ligatured larvae does not induce appearance of these 2 proteins. Accumulation of protein 3 that reacts withBombyx moriarylphorin antiserum is not blocked by juvenile hormone and is similar in both phases. It also accumulates to a small extent in the haemolymph during the moult to the final-larval instar and then disappears at ecdysis. One-hundred ng/ml ecdysteroid caused the sequestration of these proteins by the fat body, but a higher concentration of ecdysteroid (200 ng/ml) produced pupal cuticle in the isolated abdomens, suggesting that different ecdysteroid concentrations are necessary for these two events.