A Coculture Based Tyrosine-Tyrosinase Electrochemical Gene Circuit for Connecting Cellular Communication with Electronic Networks
A Coculture Based Tyrosine-Tyrosinase Electrochemical Gene Circuit for Connecting Cellular Communication with Electronic Networks
复制标题
基于共培养的酪氨酸-酪氨酸酶电化学基因电路，用于连接细胞通信与电子网络
DOI：
10.1021/acssynbio.9b00469
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发表时间：
2020
影响因子：
4.7
通讯作者：
Bentley, William E.
中科院分区：
文献类型：
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作者：
VanArsdale, Eric;Hörnström, David;Sjöberg, Gustav;Järbur, Ida;Pitzer, Juliana;Payne, Gregory F.;van Maris, Antonius J.;Bentley, William E.
人们对调解生物学和电子学之间的信息传输越来越感兴趣。通过向各种样品和细胞中添加氧化还原介体，人们既可以通过电子方式获得生物系统的氧化还原“肖像”，也可以反过来对基因表达进行编程。在这里，我们创建了一个基于细胞的合成生物学-电化学轴，其中工程细胞处理分子线索，产生可以通过电子设备直接记录的输出，但不需要添加氧化还原介体。该过程稳健；两个关键组件必须共同作用才能提供有效信号。该系统建立在酪氨酸酶介导的酪氨酸转化为左旋多巴和左旋多巴醌的基础上，这两种物质都具有氧化还原活性。 “催化”换能细胞提供信号介导的酪氨酸酶表面表达。此外，“试剂”转导细胞合成并输出酪氨酸，酪氨酸是酪氨酸酶的底物。在共培养中，该系统能够实现细胞激活分子线索的实时电化学转导。为了证明这一点，我们窃听了铜绿假单胞菌、N-(3-氧代十二酰基)-l-高丝氨酸内酯和绿脓素分泌的群体感应信号分子。
There is a growing interest in mediating information transfer between biology and electronics. By the addition of redox mediators to various samples and cells, one can both electronically obtain a redox “portrait” of a biological system and, conversely, program gene expression. Here, we have created a cell-based synthetic biology–electrochemical axis in which engineered cells process molecular cues, producing an output that can be directly recorded via electronics—but without the need for added redox mediators. The process is robust; two key components must act together to provide a valid signal. The system builds on the tyrosinase-mediated conversion of tyrosine to L-DOPA and L-DOPAquinone, which are both redox active. “Catalytic” transducer cells provide for signal-mediated surface expression of tyrosinase. Additionally, “reagent” transducer cells synthesize and export tyrosine, a substrate for tyrosinase. In cocultures, this system enables real-time electrochemical transduction of cell activating molecular cues. To demonstrate, we eavesdrop on quorum sensing signaling molecules that are secreted byPseudomonas aeruginosa,N-(3-oxododecanoyl)-l-homoserine lactone and pyocyanin.