Cloned T lymphocytes and monoclonal antibodies as probes for cell surface molecules active in T cell-mediated cytolysis.
Cloned T lymphocytes and monoclonal antibodies as probes for cell surface molecules active in T cell-mediated cytolysis.
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克隆 T 淋巴细胞和单克隆抗体作为 T 细胞介导的细胞溶解中活跃的细胞表面分子的探针。
DOI：
10.1111/j.1600-065x.1982.tb01063.x
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发表时间：
1982
影响因子：
8.7
通讯作者：
Fitch,FW
中科院分区：
文献类型：
--
作者：
Sarmiento,M;Dialynas,DP;Lancki,DW;Wall,KA;Lorber,MI;Loken,MR;Fitch,FW
特异性溶细胞性T淋巴细胞（CTL）的出现是对病毒感染和同种异体移植物的细胞介导的免疫应答的显著特征。CTL通过由至少三个步骤组成的过程杀死它们的靶细胞：（1）抗原识别和细胞粘附，（2）Ca 2+依赖性致死性命中，和（3）靶细胞死亡。在T细胞介导的细胞溶解中观察到的抗原识别的高度特异性性质表明，必须涉及有区别的抗原受体分子，但鉴定这种T细胞受体的尝试只取得了有限的成功。除了抗原特异性受体之外，其他细胞表面分子似乎参与细胞溶解过程。与细胞表面组分反应的抗血清已被用于尝试确定参与细胞溶解的分子。几个研究者（Nakayama等，1979，Shinohara & Sachs 1979）证明抗Lyl-2同种抗血清可以抑制由从免疫动物或从混合淋巴细胞培养物（MLC）获得的T细胞介导的细胞溶解。然而，这些结果的解释由于这样的抗血清可能含有与其他抗原反应的抗体而变得复杂。此外，
The appearance of specific cytolytic T lymphocytes (CTL) is a prominent feature of cell-mediated immune responses to viral infections and to aliografts. CTL kill their target cells through a process consisting of at least three steps:(1) antigen recognition and celhceil adherence,(2) a Ca^-dependent lethal hit, and (3) target cell death. The highly specific nature of the antigenic recognition observed in T cell-mediated cytolysis indicates that a discriminating antigenreceptor molecule must be Involved, but attempts to identify this T cell receptor have met with only limited success. In addition to an antigen-specific receptor, other cell surface molecules appear to participate in the cytoiytic process. Antisera reactive with cell surface components have been utilized in attempts to define the molecules involved in cytolysis. Several investigators (Nakayama et al. 1979, Shinohara & Sachs 1979) demonstrated that anti-Lyl-2 alloantisera couid inhibit cytolysis mediated by T cells obtained from immunized animals or from mixed lymphocyte cultures (MLC). However, the interpretation of these results was complicated by the fact that such antisera may have contained antibodies which reacted with other antigens. In addition, it was difficult to be