Chronic Infusion of Astaxanthin Into Hypothalamic Paraventricular Nucleus Modulates Cytokines and Attenuates the Renin-Angiotensin System in Spontaneously Hypertensive Rats
Chronic Infusion of Astaxanthin Into Hypothalamic Paraventricular Nucleus Modulates Cytokines and Attenuates the Renin-Angiotensin System in Spontaneously Hypertensive Rats
复制标题
虾青素长期注入下丘脑室旁核可调节自发性高血压大鼠的细胞因子并减弱肾素-血管紧张素系统
DOI：
10.1097/fjc.0000000000000953
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发表时间：
2021-02-01
影响因子：
3
通讯作者：
Kang, Yu-Ming
中科院分区：
文献类型：
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作者：
Gao, Hong-Li;Yu, Xiao-Jing;Kang, Yu-Ming
氧化应激、肾素-血管紧张素系统（RAS）和炎症是高血压发病机制中的一些机制。本研究的目的是检查从螃蟹和虾壳中提取的虾青素长期给药到自发性高血压大鼠下丘脑室旁核（PVN）的保护作用。将动物随机分配到2组，并通过渗透微型泵（Alzet渗透泵，2004型，0.25 μ L/h）用虾青素或载体（人工脑脊液）的双侧PVN输注治疗4周。与Wistar-Kyoto大鼠相比，自发性高血压大鼠的平均动脉压和血浆去甲肾上腺素和促炎细胞因子水平较高; PVN活性氧、NOX 2、NOX 4、IL-1 β、IL-6、ACE和AT 1-R水平较高; PVN IL-10和Cu/Zn SOD、Mn SOD、ACE 2和Mas受体水平较低。我们的数据表明，长期给予虾青素到PVN减弱了PVN内活性氧、NOX 2、NOX 4、炎性细胞因子和RAS组分的过度表达，并抑制了高血压。目前的研究结果表明，虾青素在大脑中发挥作用。我们的研究结果表明，虾青素通过改善炎症细胞因子和RAS组分之间的平衡对高血压具有保护作用。
Oxidative stress, the renin-angiotensin system (RAS), and inflammation are some of the mechanisms involved in the pathogenesis of hypertension. The aim of this study is to examine the protective effect of the chronic administration of astaxanthin, which is extracted from the shell of crabs and shrimps, into hypothalamic paraventricular nucleus (PVN) in spontaneously hypertensive rats. Animals were randomly assigned to 2 groups and treated with bilateral PVN infusion of astaxanthin or vehicle (artificial cerebrospinal fluid) through osmotic minipumps (Alzet Osmotic Pumps, Model 2004, 0.25 mu L/h) for 4 weeks. Spontaneously hypertensive rats had higher mean arterial pressure and plasma level of norepinephrine and proinflammatory cytokine; higher PVN levels of reactive oxygen species, NOX2, NOX4, IL-1 beta, IL-6, ACE, and AT1-R; and lower PVN levels of IL-10 and Cu/Zn SOD, Mn SOD, ACE2, and Mas receptors than Wistar-Kyoto rats. Our data showed that chronic administration of astaxanthin into PVN attenuated the overexpression of reactive oxygen species, NOX2, NOX4, inflammatory cytokines, and components of RAS within the PVN and suppressed hypertension. The present results revealed that astaxanthin played a role in the brain. Our findings demonstrated that astaxanthin had protective effect on hypertension by improving the balance between inflammatory cytokines and components of RAS.