Polyphyletic origin of cultivated rice: Based on the interspersion pattern of SINEs
Polyphyletic origin of cultivated rice: Based on the interspersion pattern of SINEs
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DOI：
10.1093/molbev/msg004
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发表时间：
2003-01-01
影响因子：
10.7
通讯作者：
Ohtsubo, E
中科院分区：
文献类型：
--
作者：
Cheng, CY;Motohashi, R;Ohtsubo, E
野生稻具有较大的种内变异，被认为是具有两种生态型的栽培稻的祖先。为了确定栽培稻的起源，研究了水稻反转录基因p-SINE1的亚家族成员，这些成员在栽培稻中表现出插入多态。对栽培稻和野生稻的101个品系进行了鉴定，并根据各自基因座上有无p-SINE1成员的情况对每个品系进行了条形码分析。根据水稻品系的条形码构建的系统发育树表明，野生稻可分为两大类群，分别对应于水稻和水稻，而普通野生稻可分为4个类群，其中一年生野生稻与其他3个类群的多年生野生稻不同。亚种间的亲缘关系较近，亚种间的亲缘关系较近，表明普通野生稻与多年生野生稻有较近的亲缘关系，表明普通野生稻与普通野生稻有多系统关系。即使人们对水稻品系的形态、分类、生理和生化了解有限，p-SINE1亚家族成员仍然是研究水稻品系分类和亲缘关系的有力工具。
The wild rice species Oryza rufipogon with wide intraspecific variation is thought to be the progenitor of the cultivated rice species Oryza sativa with two ecotypes, japonica and indica. To determine the origin of cultivated rice, subfamily members of the rice retroposon p-SINE1, which show insertion polymorphism in the O. sativa -O. rufipogon population, were identified and used to "bar code" each of 101 cultivated and wild rice strains based on the presence or absence of the p-SINE1 members at the respective loci. A phylogenetic tree constructed based on the bar codes given to the rice strains showed that O. sativa strains were classified into two groups corresponding to japonica and indica, whereas O. rufipogon strains were in four groups, in which annual O. rufipogon strains formed a single group, differing from the perennial O. rufipogon strains of the other three groups. Japonica strains were closely related to the O. rufipogon perennial strains of one group, and the indica strains were closely related to the O. rufipogon annual strains, indicating that O. sativa has been derived polyphyletically from O. rufipogon. The subfamily members of p-SINE1 constitute a powerful tool for studying the classification and relationship of rice strains, even when one has limited knowledge of morphology, taxonomy, physiology, and biochemistry of rice strains.