An SEM analysis of neural crest migration in the mouse.
An SEM analysis of neural crest migration in the mouse.
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发表时间：
1983-04
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影响因子：
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通讯作者：
C. Erickson;J. A. Weston
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作者：
C. Erickson;J. A. Weston
描述了正常小鼠胚胎中主干神经嵴的细胞形态和迁移途径，以及神经嵴衍生物发育异常的Patch纯合子胚胎。干神经嵴细胞最初出现在8天半的胚胎中，是一个独特的细胞群，位于背神经管表面，相对圆形。一旦它们开始迁移，细胞就会变平，并在一定程度上与神经管切向，并在体体和神经管之间向前移动。在迁移开始时，神经嵴细胞将板足延伸到管表面，同时将它们的尾突从管腔表面分离。当细胞在此区域开始迁移时，背神经管上的基板是不连续的。随着发育的进行，基底板逐渐从外侧向背侧连续，神经嵴迁移逐渐局限于不连续的基底板变窄区域。细胞与神经管的分离随着连续基底膜的形成而停止。对突变胚胎的初步观察表明，细胞外空间出现异常，嵴迁移模式随后发生改变。我们认为细胞外基质、细胞外间隙和基底膜可能决定了小鼠的嵴迁移。
The cellular morphology and migratory pathways of the trunk neural crest are described in normal mouse embryos, and in embryos homozygous for Patch in which neural crest derivatives develop abnormally. Trunk neural crest cells initially appear in 8 1/2-day embryos as a unique cell population on the dorsal neural tube surface and are relatively rounded. Once they begin to migrate the cells flatten and orient somewhat tangentially to the neural tube, and advance ventrad between the somites and neural tube. At the onset of migration neural crest cells extend lamellipodia onto the surface of the tube while detaching their trailing processes from the lumenal surface. The basal lamina on the dorsal neural tube is discontinuous when cell migration begins in this region. As development proceeds, the basal lamina gradually becomes continuous from a lateral to dorsal direction and neural crest emigration is progressively confined to the narrowing region of discontinuous basal lamina. Cell separation from the neural tube ceases concomitant with completion of a continuous basement membrane. Preliminary observations of the mutant embryos reveal that abnormal extracellular spaces appear and patterns of crest migration are subsequently altered. We conclude that the extracellular matrix, extracellular spaces and basement membranes may delimit crest migration in the mouse.