Genistein, daidzein and glycitein inhibit growth and DNA synthesis of aortic smooth muscle cells from stroke-prone spontaneously hypertensive rats
Genistein, daidzein and glycitein inhibit growth and DNA synthesis of aortic smooth muscle cells from stroke-prone spontaneously hypertensive rats
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DOI：
10.1093/jn/131.4.1154
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发表时间：
2001-04-01
影响因子：
4.2
通讯作者：
Yamori, Y
中科院分区：
文献类型：
--
作者：
Pan, W;Ikeda, K;Yamori, Y
最近的研究报道，雌激素替代疗法（ERT）降低绝经后妇女心血管疾病的风险。然而，这种效应的机制尚未完全了解，ERT与女性致癌副作用和男性女性化效应有关。由于大豆异黄酮是一组天然植物雌激素，只有微弱的雌激素活性，并没有已知的副作用，如致癌性和女性化，我们评估了染料木黄酮，大豆黄酮和黄豆黄素对主动脉平滑肌细胞（SMC）的生长和DNA合成的影响从易卒中的自发性高血压大鼠（SHRSP）。SMC在培养皿中培养，并在10%葡聚糖包被的活性炭/胎牛血清上增殖，然后用0.1-30 μ mol/L的染料木黄酮、大豆黄酮或黄豆黄素处理，以分别研究细胞增殖（细胞数）和DNA合成（细胞增殖ELISA系统）。我们还研究了它们对血小板衍生生长因子（PDGF）-BB（20 μ g/L）诱导的SMC增殖的影响。大豆异黄酮呈浓度依赖性抑制SHRSP平滑肌细胞增殖和DNA合成。在3 μ mol/L的染料木素和10 μ mol/L的大豆黄酮和黄豆黄素的抑制是显着的。对于PDGF-BB诱导的SMC增殖的显著抑制，低至0.1 μ mol/L的每种浓度是有效的。这些甾体类化合物对天然和PDGF-BB诱导的SMC增殖具有抑制作用，可能有助于减弱这种增殖，这是动脉粥样硬化血管变化的基本机制，从而预防动脉粥样硬化性心血管疾病。
Recent studies have reported that estrogen replacement therapy (ERT) reduces the risk of cardiovascular diseases in postmenopausal women. However, mechanisms responsible for this effect are not yet completely understood, and ERT is associated with carcinogenic side effects in women and feminizing effects in men. Because soybean isoflavones, a group of natural phytoestrogens, have only weak estrogenic activity and are not known to have side effects such as carcinogenesis and feminization, we evaluated the effects of genistein, daidzein and glycitein on the growth and DNA synthesis of aortic smooth muscle cells (SMC) from stroke-prone spontaneously hypertensive rats (SHRSP). SMC were cultured in dishes and proliferated on 10% dextran-coated charcoal/fetal bovine serum, and then treated with 0.1-30 mu mol/L of genistein, daidzein or glycitein to investigate cell proliferation (cell number) and DNA synthesis (cell proliferation ELISA system), respectively. We also studied their effects on platelet-derived growth factor (PDGF)-BB (20 mug/L)-induced SMC proliferation. Soybean isoflavones inhibited proliferation and DNA synthesis of SMC from SHRSP in a concentration-dependent manner. Inhibition was significant at 3 mu mol/L of genistein and 10 mu mol/L of both daidzein and glycitein. For significant inhibition of PDGF-BB-induced SMC proliferation, concentrations as low as 0.1 mu mol/L of each isoflavone were effective. These isoflavones, with their inhibitory effects on natural and PDGF-BB-induced SMC proliferation, may be useful in attenuatating such proliferation, a basic mechanism involved in atherosclerotic vascular change, thereby preventing atherosclerotic cardiovascular diseases.