Gut microbial functional maturation and succession during human early life
Gut microbial functional maturation and succession during human early life
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DOI：
10.1111/1462-2920.14235
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发表时间：
2018-06-01
影响因子：
5.1
通讯作者：
Campoy, Cristina
中科院分区：
文献类型：
--
作者：
Cerdo, Tomas;Ruiz, Alicia;Campoy, Cristina
从出生起肠道微生物定植的进化轨迹已被证明是以后健康的最好条件。在这里，我们结合与培养无关的16S rRNA基因测序和代谢蛋白质组学来研究6个月和18个月大的健康足月婴儿粪便样本中肠道微生物区系的功能成熟。对代谢蛋白质组的系统发育分析表明，双歧杆菌提供了最多数量的不同蛋白质组。在所有的分类级别上，在分类单元丰度和蛋白质系统发育上都观察到了相当大的差异。年龄对早期微生物区系有深刻的影响，在那里，差异较小的群落的组成和功能多样性随着时间的推移而增加。蛋白质相对丰度的比较揭示了分类群相关的糖解和发酵策略的转变，从牛奶和粘蛋白来源的单糖分解代谢提供醋酸盐/丙酸合成，到复杂的食物来源的己糖提供丁酸合成。此外，共生网络分析揭示了功能类群的两个反相关模块。一个以双歧杆菌科为中心的低连接兼性厌氧菌协会被一个丰富的专性厌氧菌俱乐部所取代，这些专性厌氧菌在乳螺科周围紧密相连，支撑着它们在微生物生态系统组装中的关键作用。我们的发现建立了一个框架，以可视化整个微生物群落新陈代谢和生态系统演替动态，为生命早期以微生物区系为目标的健康促进战略提供机会。
The evolutional trajectory of gut microbial colonization from birth has been shown to prime for healthlater in life. Here, we combined cultivation-independent 16S rRNA gene sequencing and metaproteomics to investigate the functional maturation of gut microbiota in faecal samples from full-term healthy infants collected at 6 and 18 months of age. Phylogenetic analysis of the metaproteomes showed that Bifidobacterium provided the highest number of distinct protein groups. Considerable divergences between taxa abundance and protein phylogeny were observed at all taxonomic ranks. Age had a profound effect on early microbiota where compositional and functional diversity of less dissimilar communities increased with time. Comparisons of the relative abundances of proteins revealed the transition of taxon-associated saccharolytic and fermentation strategies from milk and mucin-derived monosaccharide catabolism feeding acetate/propanoate synthesis to complex food-derived hexoses fuelling butanoate production. Furthermore, co-occurrence network analysis uncovered two anti-correlated modules of functional taxa. A low-connected Bifidobacteriaceae-centred guild of facultative anaerobes was succeeded by a rich club of obligate anaerobes densely interconnected around Lachnospiraceae, underpinning their pivotal roles in microbial ecosystem assemblies. Our findings establish a framework to visualize whole microbial community metabolism and ecosystem succession dynamics, proposing opportunities for microbiota-targeted health-promoting strategies early in life.