Systemically Transplanted Bone Marrow-derived Cells Contribute to Dental Pulp Regeneration in a Chimeric Mouse Model.
Systemically Transplanted Bone Marrow-derived Cells Contribute to Dental Pulp Regeneration in a Chimeric Mouse Model.
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DOI：
10.1016/j.joen.2015.10.007
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发表时间：
2016-02
影响因子：
4.2
通讯作者：
Wen'an Xu;Shan Jiang;Qiuyue Chen;Yanyan Ye;Jiajing Chen;B. Heng;Q. Jiang;Buling Wu;Z. Ding;Chengfei Zhang
中科院分区：
文献类型：
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作者：
Wen'an Xu;Shan Jiang;Qiuyue Chen;Yanyan Ye;Jiajing Chen;B. Heng;Q. Jiang;Buling Wu;Z. Ding;Chengfei Zhang
前言通过血液循环的迁移细胞或根尖细胞可能参与牙髓组织的再生或更新。本研究调查是否全身移植骨髓细胞可以有助于牙髓再生嵌合mouse model.MethodsA嵌合小鼠模型，通过骨髓细胞注射绿色荧光蛋白（GFP）转基因C57 BL/6小鼠的受体野生型C57 BL/6小鼠，已被照射致死剂量为8.5戈伊从高频直线加速器的尾静脉。对这些小鼠进行牙髓切除术和牙髓血运重建术。在1，4，和8周后，在vivoanimal成像和组织学analysiswere carried.ResultsIn vivoanimal成像显示，从移植的GFP+细胞的绿色生物荧光信号显着增加，并保持在一个高水平，在手术后的第一个4周。术后8周牙体标本的免疫荧光分析显示，再生的牙髓样组织中存在nestin+/GFP+、α-SMA/GFP+和NeuN/GFP+细胞。
IntroductionMigratory cells via blood circulation or cells adjacent to the root apex may potentially participate in dental pulp tissue regeneration or renewal. This study investigated whether systemically transplanted bone marrow cells can contribute to pulp regeneration in a chimeric mouse model.MethodsA chimeric mouse model was created through the injection of bone marrow cells from green fluorescent protein (GFP) transgenic C57BL/6 mice into the tail veins of recipient wild-type C57BL/6 mice that had been irradiated with a lethal dose of 8.5 Gy from a high-frequency linear accelerator. These mice were subjected to pulpectomy and pulp revascularization. At 1, 4, and 8 weeks after surgery,in vivoanimal imaging and histologic analyses were conducted.ResultsIn vivoanimal imaging showed that the green biofluorescence signal from the transplanted GFP+ cells increased significantly and was maintained at a high level during the first 4 weeks after surgery. Immunofluorescence analyses of tooth specimens collected at 8 weeks postsurgery showed the presence of nestin+/GFP+, α smooth muscle actin (α-SMA)/GFP+, and NeuN/GFP+ cells within the regenerated pulplike tissue.ConclusionsThese data confirm that transplanted bone marrow–derived cells can contribute to dental pulp regeneration.