Fluorescence turn on detection of bilirubin using Fe (III) modulated BSA stabilized copper nanocluster; A mechanistic perception
Fluorescence turn on detection of bilirubin using Fe (III) modulated BSA stabilized copper nanocluster; A mechanistic perception
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DOI：
10.1016/j.aca.2018.05.026
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发表时间：
2018-11-15
影响因子：
6.2
通讯作者：
George, Sony
中科院分区：
文献类型：
--
作者：
Jayasree, M.;Aparna, R. S.;George, Sony
高胆红素血症是指体内胆红素超过正常浓度（新生儿为19.80 mg/mL，成人为1.19 x 10(-2) mg/mL）的情况。新生儿胆红素脑病可能导致不可逆的神经系统疾病。目前检测胆红素的方法的准确性受到影响。在目前的工作中，通过一锅微波辅助方法合成了牛血清白蛋白稳定的铜纳米簇（BSA-CuNCs）作为胆红素的启动检测器。合成的 BSA-CuNC 尺寸小于 4 nm，在 405 nm 处呈现亮蓝色发射。有趣的是，在较宽的 pH 范围 (1-11) 或高离子条件下，没有观察到荧光发射的明显变化。然而，Fe3+ 与 BSA-CuNC 的相互作用会引起荧光猝灭。此外，通过在其他可能共存的生物分子上添加胆红素可以重新获得荧光。基于 BSA-CuNC 的开启探针观察到良好的线性，检测限 (LoD) 为 6.62 nM。此外，对人血清和尿液进行了真实样品分析，显示出良好的回收率。 (C) 2018 Elsevier B.V. 保留所有权利。
Hyperbilirubinemia is the condition when bilirubin exceeds normal concentration in body (19.80 mg/mL in newborns and 1.19 x 10(-2) mg/mL in adults). Bilirubin encephalopathy in newborns may cause irreversible neurological disorders. Current methods for detection of bilirubin suffer from compromising accuracy. In the present work, bovine serum albumin stabilized copper nanocluster (BSA-CuNCs) was synthesized via a one pot microwave assisted method as a turn on detector for bilirubin. The synthesized BSA-CuNCs having size less than 4 nm, exhibited bright blue emission at 405 nm. Interestingly, no observable change in fluorescence emission was noticed over a wide pH range (1-11) or at high ionic conditions. However, the interaction of Fe3+ with BSA-CuNCs induces quenching of fluorescence. Moreover, the fluorescence can be regained by the addition of bilirubin over other possible coexisting biomolecules. A good linearity was observed for BSA-CuNCs based turn on probe with a Limit of Detection (LoD) 6.62 nM. Furthermore, real sample analyses were carried out with human serum and urine which showed good recovery percentage. (C) 2018 Elsevier B.V. All rights reserved.