Anaerobic elemental sulfur reduction by fungus Fusarium oxysporum
Anaerobic elemental sulfur reduction by fungus Fusarium oxysporum
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DOI：
10.1271/bbb.70083
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发表时间：
2007-10-01
影响因子：
1.6
通讯作者：
Takaya, Naoki
中科院分区：
文献类型：
--
作者：
Abe, Tsuyoshi;Hoshino, Takayuki;Takaya, Naoki
研究了尖孢镰刀菌对无机硫化合物的还原作用。当从常氧环境转入缺氧环境时，F.尖孢菌将元素硫还原成硫化氢（H2S）。该反应伴随真菌生长和碳源（乙醇）氧化成乙酸盐。产生的H2S是乙酸盐的2倍以上，这是乙醇氧化成乙酸盐的理论相关性。NADH依赖的硫还原酶（SR）的活性检测在无细胞提取物的H2S生产的真菌，并被发现在厌氧条件下上调。另一方面，细胞的O-2消耗和粗线粒体组分的细胞色素c氧化酶活性下降。这些结果表明，SR参与H2S的产生是由于F的一种新的异化机制。oxysporum，并且该真菌通过用硫还原代替O-2呼吸的能量产生机制来适应厌氧条件。
Reduction of inorganic sulfur compounds by the fungus Fusarium oxysporum was examined. When transferred from a normoxic to an anoxic environment, F. oxysporum reduced elemental sulfur to hydrogen sulfide (H2S). This reaction accompanied fungal growth and oxidation of the carbon source (ethanol) to acetate. Over 2-fold more of H2S than of acetate was produced, which is the theoretical correlation for the oxidation of ethanol to acetate. NADH-dependent sulfur reductase (SR) activity was detected in cell-free extracts of the H2S-producing fungus, and was found to be up-regulated under the anaerobic conditions. On the other hands both O-2 consumption by the cells and cytochrome c oxidase activity by the crude mitochondrial fractions decreased. These results indicate that H2S production involving SR was due to a novel dissimilation mechanism of F. oxysporum, and that the fungus adapts to anaerobic conditions by replacing the energy-producing mechanism of O-2 respiration with sulfur reduction.