The influence of serum-supplemented culture media in a transwell migration assay
The influence of serum-supplemented culture media in a transwell migration assay
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DOI：
10.1002/cbin.11122
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发表时间：
2019-07-16
影响因子：
3.9
通讯作者：
Vidyadaran, Sharmili
中科院分区：
文献类型：
--
作者：
Zaki, Siti Sarah Omar;Kanesan, Livashini;Vidyadaran, Sharmili
Our work cautions against the use of serum-supplemented culture media in a transwell migration assay when using chemoattractants other than FBS. At 24 h, a 5% foetal bovine serum (FBS) gradient caused BV2 microglia to migrate toward the lower compartment of the transwell apparatus. Interestingly, FBS-supplemented media in the absence of a gradient also resulted in notable microglia migration. Serum can therefore confound the interpretation of a transwell migration assay when another chemoattractant is used.
