Increase in glutamine-non-amidated muropeptides in the peptidoglycan of vancomycin-resistant Staphylococcus aureus strain Mu50
Increase in glutamine-non-amidated muropeptides in the peptidoglycan of vancomycin-resistant Staphylococcus aureus strain Mu50
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DOI：
10.1093/jac/42.3.315
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发表时间：
1998-09-01
影响因子：
5.2
通讯作者：
Hiramatsu, K
中科院分区：
文献类型：
--
作者：
Hanaki, H;Labischinski, H;Hiramatsu, K
比较万古霉素耐药金黄色葡萄球菌（VRSA）Mu 50株（MIC 8 mg/L）和异源VRSA Mu 3株（MIC 3 mg/L）的肽聚糖组成，以了解万古霉素耐药的机制。与Mu 3相比，Mu 50的细胞壁具有增加量的谷氨酰胺-非酰胺化的胞肽和减少的肽聚糖交联，其中胞肽的二聚体/单体比率大大降低。与该观察结果一致，Mu 50的肽聚糖结合的万古霉素比Mu 3的多1.4倍，非酰胺化胞肽的增加和细胞壁肽聚糖交联的减少可能通过增加前代谢产物对万古霉素的消耗而导致万古霉素耐药性。存在的Mu 50细胞壁并减少到达抗生素的重要靶标所在的细胞质膜的万古霉素的量。
The peptidoglycan compositions of vancomycin-resistant Staphylococcus aureus (VRSA) strain Mu50 (MIC 8 mg/L) and hetero-VRSA strain Mu3 (MIC 3 mg/L) were compared in order to understand the mechanism of vancomycin resistance. As compared with Mu3, the cell wall of Mu50 had increased amounts of glutamine-non-amidated muropeptides and decreased crosslinking of peptidoglycan with a greatly decreased dimer/monomer ratio of muropeptides, In agreement with this observation, the peptidoglycan of Mu50 bound 1.4 times more vancomycin than that of Mu3, The increase in non-amidated muropeptides and the reduced crosslinking of the cell-wall peptidoglycan may contribute to the vancomycin resistance by increasing the consumption of vancomycin by the pre-existing cell wall of Mu50 and reducing the amount of vancomycin reaching the cytoplasmic membrane where the vital targets of the antibiotic are situated.