CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens.
CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens.
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DOI：
10.1093/nar/gky354
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发表时间：
2018-07-02
影响因子：
14.9
通讯作者：
Haeussler M
中科院分区：
文献类型：
--
作者：
Concordet JP;Haeussler M
CRISPOR.org是使用CRISPR-Cas9系统进行基因组编辑实验的网络工具。它在输入序列中找到指导RNA，并根据不同的分数对它们进行排名，这些分数评估感兴趣的基因组中潜在的脱靶并预测靶向活性。基因组列表不断扩大，在过去两年中增加了150多个基因组。CRISPOR试图提供一个全面的解决方案，从选择，克隆和表达指导RNA，以及提供测试指导活性和潜在脱靶所需的引物。最近的发展包括全基因组CRISPR和饱和筛选的批量设计，创建用于指导克隆的定制寡核苷酸以及设计下一代测序引物以测试脱靶突变。CRISPOR可从http://crispor.org获得，包括网站的完整源代码和独立的命令行版本。
CRISPOR.org is a web tool for genome editing experiments with the CRISPR–Cas9 system. It finds guide RNAs in an input sequence and ranks them according to different scores that evaluate potential off-targets in the genome of interest and predict on-target activity. The list of genomes is continuously expanded, with more 150 genomes added in the last two years. CRISPOR tries to provide a comprehensive solution from selection, cloning and expression of guide RNA as well as providing primers needed for testing guide activity and potential off-targets. Recent developments include batch design for genome-wide CRISPR and saturation screens, creating custom oligonucleotides for guide cloning and the design of next generation sequencing primers to test for off-target mutations. CRISPOR is available from http://crispor.org, including the full source code of the website and a stand-alone, command-line version.