Clinical reactogenicity of intradermal bacille Calmette-Guerin vaccination
Clinical reactogenicity of intradermal bacille Calmette-Guerin vaccination
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DOI：
10.1086/515201
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发表时间：
1999-04-01
影响因子：
11.8
通讯作者：
Carey, M
中科院分区：
文献类型：
--
作者：
Hoft, DF;Leonardi, C;Carey, M
在用卡介苗（BCG）皮内接种的志愿者中评价临床、微生物学和免疫学应答。大多数志愿者（98%）发展出溃疡性病变，其引流的平均+/- SE为4.3 +/- 0.29周，通过聚合酶链反应检测分枝杆菌DNA-在接种后2周BCG溃疡活检标本和接种后3天血液标本中，接种后2个月，从溃疡引流液中培养分枝杆菌，证明疫苗株接触传播的潜在风险延长。溃疡引流持续时间与接种前淋巴组织增生性淋巴细胞减少呈负相关。（r = -0.515; P < .002）和干扰素γ（r = -0.841; P < .002）对分枝杆菌的特异性反应，与接种后淋巴增殖性淋巴细胞增加直接相关。（r = 0.498; P <0.002）和干扰素γ（r = 0.688; P <0.02）对分枝杆菌的特异性反应。这些结果证明了BCG的临床反应原性和疫苗株接触传播的潜在风险，并表明临床反应原性是诱导保护性分枝杆菌免疫的权衡。
Clinical, microbiological, and immunologic responses were evaluated in volunteers vaccinated intradermally with bacille Calmette-Guerin (BCG), Most volunteers (98%) developed ulcerative lesions that drained for a mean +/- SE of 4.3 +/- 0.29 weeks, Mycobacterial DNA was detected by a polymerase chain reaction-based amplification technique in biopsy specimens from BCG ulcers 2 weeks after vaccination and in blood specimens 3 days after vaccination. Mycobacteria were cultured from ulcer drainage 2 months after vaccination, demonstrating a prolonged potential risk of contact spread of the vaccine strain. The duration of ulcer drainage was inversely correlated with prevaccination lymphoproliferative (r = -0.515; P < .002) and interferon gamma (r = -0.841; P < .002) responses specific to mycobacteria and directly correlated with postvaccination increases in lymphoproliferative (r = 0.498; P < .002) and interferon gamma (r = 0.688; P < .02) responses specific to mycobacteria These results demonstrate the clinical reactogenicity of BCG and the potential risk of contact spread of the vaccine strain and suggest that clinical reactogenicity is a trade-off for the induction of protective mycobacterial immunity.