Transformation of tracheal epithelium exposed in vitro to N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG)
Transformation of tracheal epithelium exposed in vitro to N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG)
复制标题
体外暴露于 N-甲基-N-硝基-N-亚硝基胍 (MNNG) 的气管上皮的转化
DOI：
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发表时间：
1977
影响因子：
6.4
通讯作者：
P. Nettesheim
中科院分区：
文献类型：
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作者：
V. Steele;A. Marchok;P. Nettesheim
利用器官培养/细胞培养系统，我们在体外转化大鼠气管上皮细胞暴露于MNNG。将每组10个气管器官培养物暴露于0、0.001、1.0或10.0 μg MNNG/ml培养基中两次（在第3天和第6天）。暴露后，将外植体置于培养皿底部，以启动上皮细胞生长并建立原代培养物。将每个外植体再植8-10次以产生多个生长物。获得的原代培养物数量和随后建立的细胞系数量具有致癌物剂量依赖性。暴露于0、0.001、1.0和10.0 μg MNNG/ml后，每个外植体的原代上皮细胞培养物平均数分别为1.3、1.5、3.3和4.6。在这些组中每个外植体的细胞系的平均产量分别为0、0.8、1.3和2.0。很明显，细胞系只能从暴露于致癌物的上皮细胞中建立。目前正在对这些细胞系进行体内致瘤性测试。迄今为止，在第5代和第15代之间检测的35个细胞系中，10 μg MNNG/ml组的5个细胞系和1.0 μg MNNG/ml组的2个细胞系在注射到免疫抑制受体后产生了可触及的肿瘤。
Using an organ culture/cell culture system, we transformed rat tracheal epithelial cells in vitro by exposure to MNNG. Ten tracheal organ cultures per group were exposed twice (at days 3 and 6) to 0,0.001, 1.0 or 10.0 μg MNNG/ml of medium. Following this exposure, the explants were placed on the bottom of culture dishes to initiate epithelial cell outgrowths and establish primary cultures. Each explant was replanted 8–10 times to produce multiple outgrowths. The number of primary cultures and the number of subsequently established cell lines obtained was carcinogen‐dose‐dependent. The average number of primary epithelial cell cultures per explant after exposure to 0, 0.001, 1.0 and 10.0 μg MNNG/ml was 1.3, 1.5, 3.3, and 4.6, respectively. The average yield of cell lines per explant in these groups was 0, 0.8, 1.3, and 2.0, respectively. It is apparent that cell lines could only be established from carcinogen‐exposed epithelium. These cell lines are currently being tested for tumorigenicity in vivo. To date, of 35 cell lines tested between the 5th and 15th passages, 5 cell lines from the 10 μg MNNG/ml group and 2 from the 1.0 μg MNNG/ml group have produced palpable tumors upon injection into immuno‐suppressed recipients.