Characterization of in vitro phenotypes of Burkholderia pseudomallei and Burkholderia mallei strains potentially associated with persistent infection in mice
Characterization of in vitro phenotypes of Burkholderia pseudomallei and Burkholderia mallei strains potentially associated with persistent infection in mice
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DOI：
10.1007/s00203-016-1303-8
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发表时间：
2017-03-01
影响因子：
2.8
通讯作者：
Welkos, S. L.
中科院分区：
文献类型：
--
作者：
Bernhards, R. C.;Cote, C. K.;Welkos, S. L.
类鼻疽伯克霍尔德菌（Bp）和鼻疽伯克霍尔德菌（Bm）分别是类鼻疽和鼻疽的病原体，属于1级生物威胁。它们感染人类和动物，引起从急性和致命到长期和慢性的疾病。慢性感染的治疗尤其具有挑战性，并且识别从急性到持续性感染进展的体外表型标志物将是极其有价值的。首先，采用菌落形态分型、化学敏感性测试、巨噬细胞感染和脂多糖指纹分析来开发表型分型策略以区分伯克霍尔德菌菌株。然后在感染后3-180天收集小鼠脾脏分离物，对其进行表型表征。从长期感染的分离株往往表现出增加的菌落形态差异和改变抗菌药物敏感性和巨噬细胞感染的模式。一些Bp和Bm持续感染分离株在小鼠中清楚地显示出增强的毒力。未来的研究将评估这些表型标志物在慢性感染建立信号中的潜在作用和意义。
Burkholderia pseudomallei (Bp) and Burkholderia mallei (Bm), the agents of melioidosis and glanders, respectively, are Tier 1 biothreats. They infect humans and animals, causing disease ranging from acute and fatal to protracted and chronic. Chronic infections are especially challenging to treat, and the identification of in vitro phenotypic markers which signal progression from acute to persistent infection would be extremely valuable. First, a phenotyping strategy was developed employing colony morphotyping, chemical sensitivity testing, macrophage infection, and lipopolysaccharide fingerprint analyses to distinguish Burkholderia strains. Then mouse spleen isolates collected 3-180 days after infection were characterized phenotypically. Isolates from long-term infections often exhibited increased colony morphology differences and altered patterns of antimicrobial sensitivity and macrophage infection. Some of the Bp and Bm persistent infection isolates clearly displayed enhanced virulence in mice. Future studies will evaluate the potential role and significance of these phenotypic markers in signaling the establishment of a chronic infection.