Growth rate-dependent modulation of carbon flux through central metabolism and the kinetic consequences for glucose-limited chemostat cultures of Corynebacterium glutamicum
Growth rate-dependent modulation of carbon flux through central metabolism and the kinetic consequences for glucose-limited chemostat cultures of Corynebacterium glutamicum
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DOI：
10.1128/aem.62.2.429-436.1996
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发表时间：
1996-02-01
影响因子：
4.4
通讯作者：
Lindley, ND
中科院分区：
文献类型：
--
作者：
CocaignBousquet, M;Guyonvarch, A;Lindley, ND
从生长动力学和酶的角度研究了谷氨酸棒杆菌在葡萄糖限制恒化培养中的生理行为。根据生长动力学计算中心代谢内的代谢通量，并分析与特定酶活性的关系。在高生长速率下，观察到葡萄糖去除不完全，这归因于磷酸转移酶系统转运蛋白的限速能力和低亲和力通透酶摄取机制的可能贡献。在高生长速率下观察到的提高的生物质产量与回补羧化反应谱的转变有关，其中丙酮酸羧化酶取代苹果酸酶，磷酸烯醇丙酮酸羧化酶，这一活动通常被认为是C.谷氨酸单胞菌对葡萄糖的作用仅以低水平存在，并且除了在低生长速率下之外不太可能显著地促进三羧酸循环供能。
The physiological behavior of Corynbacterium glutanicum in glucose-limited chemostat cultures was examined from both growth kinetics and enzymatic viewpoints. Metabolic fluxes within the central metabolism were calculated from growth kinetics and analyzed in relation to specific enzyme activities, At high growth rates, incomplete glucose removal was observed, and this was attributed to rate-limiting capacity of the phosphotransferase system transporter and the probable contribution of a low-affinity permease uptake mechanism, The improved biomass yield observed at high growth rates was related to a shift in the profile of anaplerotic carboxylation reactions, with pyruvate carboxylase replacing malic enzyme, Phosphoenolpyruvate carboxylase, an activity often assumed to be the major anaplerotic reaction during growth of C. glutamicum on glucose, was present at only low levels and is unlikely to contribute significantly to tricarboxylic acid cycle fuelling other than at low growth rates.