Electrokinetic preconcentration and electrochemical detection of Escherichia coli at a microelectrode
Electrokinetic preconcentration and electrochemical detection of Escherichia coli at a microelectrode
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DOI：
10.1016/j.electacta.2018.05.120
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发表时间：
2018-08-01
影响因子：
6.6
通讯作者：
Boika, Aliaksei
中科院分区：
文献类型：
--
作者：
Frkonja-Kuczin, Ariana;Ray, Louis;Boika, Aliaksei
在本文中，我们描述了一种新的电分析方法，利用大肠杆菌（E。大肠杆菌）细菌的电动力现象（介电电泳和微流体流动），所述电动力现象是通过高频交流（AC）波形在电解质溶液中产生的。细菌被银纳米颗粒修饰，因此变得有可能逐个检测单个细菌细胞，即，进行电化学计数，由于银在工作微电极（UME）上氧化，在适当的直流电位下偏置。我们证明，开发的方法允许检测E。大肠杆菌的浓度比基于大肠杆菌的转移所能检测到的浓度小两个数量级。仅通过扩散将其转化为大肠杆菌（Sepunaru等人，生物体科学，3（2015）816-820）。(C)2018爱思唯尔有限公司版权所有
In this paper, we describe a novel electroanalytical approach that utilizes preconcentration of Escherichia coli (E. coli) bacteria by electrokinetic phenomena (dielectrophoresis and electrothermal fluid flow), which are generated in an electrolyte solution by a high frequency alternating current (ac) waveform. The bacteria are decorated with silver nanoparticles, so it becomes possible to detect individual bacterial cells one by one, i.e., perform electrochemical counting, due to silver oxidation on a working microelectrode (UME) biased at an appropriate dc potential. We demonstrate that the developed methodology allows for detection of E. coli concentrations two orders of magnitude smaller than what one could detect based on the transfer of E. coli by diffusion only (Sepunaru et al., Biomater. Sci., 3 (2015) 816-820). (C) 2018 Elsevier Ltd. All rights reserved.