Multipotent adult progenitor cell isolation and culture procedures
Multipotent adult progenitor cell isolation and culture procedures
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DOI：
10.1016/j.exphem.2006.07.013
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发表时间：
2006-11-01
影响因子：
2.6
通讯作者：
Jiang, Yuehua
中科院分区：
文献类型：
--
作者：
Breyer, April;Estharabadi, Nima;Jiang, Yuehua
我们描述了从新生至6周龄的小鼠和大鼠中分离多能成体祖细胞（MAPCs）的方法。这些细胞的维护，包括其培养，培养基配方和质量控制程序，也进行了解释。此外，方法来识别MAPCs，包括他们的表型和形态进行了讨论。(c)2006年国际实验血液学学会。爱思唯尔公司出版
We describe methods for isolation of multipotent adult progenitor cells (MAPCs) from newborn to 6-week-old mice and rats. The maintenance of these cells, including their culture, media formulas, and quality control procedures, are also explained. Additionally, ways to identify MAPCs including their phenotype and morphology are discussed. (c) 2006 International Society for Experimental Hematology. Published by Elsevier Inc.