Involvement of Hofbauer cells and maternal T cells in villitis of unknown aetiology
Involvement of Hofbauer cells and maternal T cells in villitis of unknown aetiology
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DOI：
10.1111/j.1365-2559.2008.02964.x
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发表时间：
2008-03-01
期刊：
影响因子：
6.4
通讯作者：
Kim, C. J.
中科院分区：
文献类型：
--
作者：
Kim, J-S;Romero, R.;Kim, C. J.
目的：不明原因绒毛炎(VUE)的病因、炎性细胞的来源和T细胞的免疫表型等方面的性质很耐人寻味。方法与结果：对45例VUE胎盘进行了Y染色体(DYZ1)的发色原位杂交和CD14、CD68、Ki67(均为男性新生儿)和一组T细胞抗原(CD3、CD4、CD8)的免疫组织化学检测(n=35)。所有男婴胎盘的巨噬细胞中均有来自Y染色体的CISH+信号，而淋巴细胞中无此信号，提示巨噬细胞来源于胎儿。绒毛巨噬细胞多为Ki67+，提示为增生性Hofbauer细胞。在不同产科条件的胎盘中，CD8+T细胞多于CD4+T细胞。结论：VUE的主要成分为母体CD8+T细胞和Hofbauer细胞。我们认为VUE是一种独特的炎症反应，来自两个宿主的白细胞是关键伙伴，类似于同种异体移植排斥反应或移植物抗宿主病。
Aims: The nature of villitis of unknown aetiology (VUE) is intriguing in terms of its aetiology, origin of inflammatory cells and immunophenotype of T cells involved. The aim was to determine the origin of macrophages and the immunophenotype of T lymphocytes in VUE associated with various complications of pregnancy.Methods and results: Placentas with VUE (n = 45) were studied by chromogenic in-situ hybridization (CISH) for Y chromosome (DYZ1) and immunohistochemistry for CD14, CD68, Ki67 (n = 10; all from male neonates) and a panel of T-cell antigens (CD3, CD4 and CD8) (n = 35). All of the placentas from male neonates showed CISH+ signals from Y chromosomes in the majority of macrophages, but not in lymphocytes, indicating that the macrophages were of fetal origin. Many macrophages of the affected chorionic villi were Ki67+, suggesting that they are hyperplastic Hofbauer cells. Among the lymphocytes, CD8+ T cells outnumbered CD4+ T cells in all placentas with different obstetrical conditions.Conclusions: We define primary components of VUE as maternal CD8+ T cells and hyperplastic Hofbauer cells. We propose that VUE is a unique inflammatory reaction where the leucocytes from two hosts are key partners, analogous to either allograft rejection or graft-versus-host disease.