Homotypic cell contact enhances insulin but not glucagon secretion
Homotypic cell contact enhances insulin but not glucagon secretion
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DOI：
10.1016/j.bbrc.2006.03.214
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发表时间：
2006-06-09
影响因子：
3.1
通讯作者：
Jones, Peter M.
中科院分区：
文献类型：
--
作者：
Brereton, Helen C.;Carvell, Melanie J.;Jones, Peter M.
胰岛内相互作用影响β细胞功能，并且胰岛结构的破坏导致葡萄糖诱导的胰岛素分泌减少。而再聚集改善分泌反应性。我们对MIN6细胞的研究表明，通过将β细胞配置为三维胰岛样结构，与其单层等同物相比，葡萄糖诱导的胰岛素分泌有显著改善。在本研究中。我们已经使用小鼠胰高血糖素分泌α TCl细胞系来观察同型相互作用在α细胞胰高血糖素分泌的调节中是否重要。我们发现，α TCl细胞的分泌反应没有显着差异保持单层或细胞簇。我们还发现，不同的细胞粘附分子参与了α细胞和β细胞之间的细胞相互作用。MIN6细胞表达ECAD，而α TC1细胞表达NCAM。ECAD是MIN6细胞而不是α TCl细胞形成细胞簇所必需的。而在任一细胞系中细胞簇的形成都不需要NCAM。(c)2006年爱思唯尔公司All rights reserved.
Intra-islet interactions influence beta-cell function, and disruption of islet architecture results in a reduction ill glucose-induced insulin secretion. whereas re-aggregation improves secretory responsiveness. Our Studies on MIN6 cells have shown that by configuring beta-cells as three-dimensional islet-like Structures there is a marked improvement in glucose-induced insulin secretion compared to that of their monolayer equivalents. In the present study. we have used the mouse glucagon-secreting alpha TCl cell line to see whether homotypic interactions Lire important in the regulation of glucagon secretion from alpha-cells. We found no significant difference in the secretory responses of,alpha TCl cells maintained as monolayers or as cell clusters. We also found that different cell adhesion molecules are involved in cell interactions between alpha- and beta-cells.- MIN6 cells express ECAD, whereas alpha TCl cells express NCAM. ECAD is necessary for cell Cluster formation by MIN6 cells but not by alpha TCl cells. whereas NCAM is not needed for the formation of cell clusters in either cell line. (c) 2006 Elsevier Inc. All rights reserved.