Electron transport reactions in a cytochrome c-deficient mutant of Paracoccus denitrificans.
Electron transport reactions in a cytochrome c-deficient mutant of Paracoccus denitrificans.
复制标题
脱氮副球菌细胞色素 c 缺陷突变体中的电子传递反应。
DOI：
10.1016/s0005-2728(89)80426-8
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Davies,HC
中科院分区：
文献类型：
--
作者：
Bolgiano,B;Smith,L;Davies,HC
A mutant ofParacoccus denitrificanswhich is deficient in c-type cytochromes grows aerobically with generation times similar to those obtained with a wild-type strain. Theaa3-type oxidase is functional in the mutant as judged by spectrophotometric assays of cytochromecoxidation using the membrane particles and cytochromeaa3reduction in whole cells. The cytochromecoxidase (aa3-type) of the c-less mutant oxidizes soluble cytochromecat rates equivalent to those obtained with the wild-type. NADH and succinate oxidase activities of the membrane preparations of the mutant and wild-type are also comparable in the absence of detergent treatment. Exogenous soluble cytochrome c can be both reduced by NADH- and succinate-linked systems and oxidized by cytochromeaa3present in membranes of the mutant strain. Rapid overall electron transport can occur in the c-less mutant, suggesting that reactions result from collision of diffusing complexes.
