A multielectrode microcompartment culture platform for studying signal transduction in the nervous system
A multielectrode microcompartment culture platform for studying signal transduction in the nervous system
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DOI：
10.1039/b612684g
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发表时间：
2006-12-01
期刊：
影响因子：
6.1
通讯作者：
Frazier, A. Bruno
中科院分区：
文献类型：
--
作者：
Ravula, Surendra K.;McClain, Maxine A.;Frazier, A. Bruno
本文描述了用于培养神经元电生理信号研究的微制隔室培养系统(MU-CCS)的设计、制造和表征。本文的重点是MU-CCS与培养神经元的对接过程，并论证该系统在生化介导电生理学研究中的适用性。此外，我们还表明，我们可以通过细胞外隔室应用高水平的K+离子来记录培养神经元的动作电位。最后，我们证明，我们可以在两室培养的其中一室中分离钠通道阻滞剂河豚毒素的电生理效应，同时记录两室的电生理数据。
This paper describes the design, fabrication, and characterization of a microfabricated compartmented culture system (mu-CCS) useful for electrophysiological signaling studies in cultured neurons. The focus of the paper is the process of interfacing the mu-CCS with cultured neurons and to demonstrate the applicability of the system for biochemical-mediated electrophysiological studies. Moreover, we show that we can record action potentials from cultured neurons through the extracellular compartmented application of elevated levels of K+ ions. Finally, we show that we can isolate the electrophysiological effects of the sodium channel blocker tetrodotoxin in one of the compartments of a two compartment culture while recording electrophysiological data from both compartments.