In vivo effector function of influenza virus-specific cytotoxic T lymphocyte clones is highly specific.
In vivo effector function of influenza virus-specific cytotoxic T lymphocyte clones is highly specific.
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DOI：
10.1084/jem.160.3.814
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发表时间：
1984-09-01
影响因子：
15.3
通讯作者：
Braciale, T J
中科院分区：
文献类型：
--
作者：
Lukacher, A E;Braciale, V L;Braciale, T J
针对A型流感病毒的鼠细胞毒性T淋巴细胞（CTL）克隆系在过继转移给被流感病毒致死性感染的同基因小鼠时能提供完全保护。在培养中一个亚型特异性克隆CTL所表现出的高度特异性反映在它仅能清除那些被该克隆系在体外识别的病毒亚型所感染的小鼠肺部病毒并介导其恢复的能力上。一个交叉反应性CTL克隆系能保护被两种流感病毒亚型中任何一种感染的小鼠。在同时感染两种病毒亚型的小鼠中，亚型特异性CTL克隆仅能降低被识别的病毒亚型在肺部的病毒水平，并不能阻止这些小鼠死亡。相比之下，将交叉反应性CTL克隆过继转移到同时感染两种病毒亚型的小鼠中，会导致两种亚型在肺部的滴度都降低，并促进完全恢复。这些结果直接表明CTL在实验性流感感染中是一种重要的抗病毒防御机制。此外，这些结果表明CTL在体内抗病毒效应活性的诱导和表达都具有高度特异性，因此支持CTL在体内通过直接裂解受感染细胞来发挥其抗病毒作用这一概念。
Cloned lines of murine cytotoxic T lymphocytes (CTL) directed to type A influenza virus confer complete protection upon adoptive transfer to syngeneic mice lethally infected by influenza virus. The exquisite specificity exhibited by a subtype-specific cloned CTL in culture is reflected in its capacity to eliminate pulmonary virus and mediate recovery only in those mice infected by the virus subtype recognized by this cloned line in vitro. A cross-reactive CTL cloned line protects mice infected by either of two influenza virus subtypes. In mice dually infected with two virus subtypes, the subtype-specific CTL clone only reduces lung virus levels of the recognized virus subtype and cannot prevent these mice from dying. In contrast, adoptive transfer of the cross-reactive CTL clone into mice simultaneously infected with two virus subtypes results in reduction of pulmonary titers of both subtypes and promotes complete recovery. These results directly implicate CTL as an important antiviral defense mechanism in experimental influenza infection. In addition, these results indicate that both the induction and expression of antiviral effector activity by CTL in vivo is highly specific and therefore favor the concept that CTL express their antiviral effect in vivo by direct cytolysis of infected cells.